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Science 20 September 1985:
Vol. 229. no. 4719, pp. 1202 - 1207
DOI: 10.1126/science.3929380
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Articles

Science, Vol 229, Issue 4719, 1202-1207
Copyright © 1985 by American Association for the Advancement of Science

articles

Transfectomas provide novel chimeric antibodies

SL Morrison

Methods have been developed to transfect immunoglobulin genes into lymphoid cells. The transfected genes are faithfully expressed, and assembly can occur both between the transfected and endogenous chains and between two transfected chains. Gene transfection can be used to reconstitute immunoglobulin molecules and to produce novel immunoglobulin molecules. These novel molecules can represent unique combinations of heavy and light chains; alternatively, by means of recombinant DNA technology, genes can be assembled in vitro, transfected, and expressed. The end products of such manipulations include chimeric molecules with variable regions joined to different isotypic constant regions; this is possible both within and between species. It is also possible to synthesize altered immunoglobulin molecules, as well as molecules having immunoglobulin sequences fused with nonimmunoglobulin sequences (for example, enzyme sequences).


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Monoclonal Antibodies for Treating Cancer.
R. O. Dillman (1989)
Ann Intern Med 111, 592-603
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Assembly of a functional immunoglobulin Fv fragment in Escherichia coli.
A Skerra and A Pluckthun (1988)
Science 240, 1038-1041
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