Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 13 September 1985:
Vol. 229. no. 4718, pp. 1104 - 1107
DOI: 10.1126/science.3929379
Prev | Table of Contents | Next

Articles

Science, Vol 229, Issue 4718, 1104-1107
Copyright © 1985 by American Association for the Advancement of Science

articles

Mutation to herbicide resistance maps within the psbA gene of Anacystis nidulans R2

SS Golden and R Haselkorn

A psbA gene encoding the target of photosystem II herbicide inhibition, the 32,000-dalton thylakoid membrane protein, has been cloned from a mutant of Anacystis nidulans R2, which is resistant to 3-(3,4-dichlorophenyl)-1,1-dimethylurea-(diuron). A cloned DNA fragment from within the coding region of this gene transforms wild-type cells to herbicide resistance, proving that mutation within psbA is responsible for that phenotype. The mutation consists of a single nucleotide change that replaces serine at position 264 of the wild-type protein with alanine in that of the diuron-resistant mutant.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Novel psbA1 Gene from a Naturally Occurring Atrazine-Resistant Cyanobacterial Isolate.
K. Sajjaphan, N. Shapir, A. K. Judd, L. P. Wackett, and M. J. Sadowsky (2002)
Appl. Envir. Microbiol. 68, 1358-1366
   Abstract »    Full Text »    PDF »
Genome rearrangement and nitrogen fixation in Anabaena blocked by inactivation of xisA gene.
J. Golden and D. Wiest (1988)
Science 242, 1421-1423
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)