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Science 13 September 1985:
Vol. 229. no. 4718, pp. 1104 - 1107
DOI: 10.1126/science.3929379
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Articles

Science, Vol 229, Issue 4718, 1104-1107
Copyright © 1985 by American Association for the Advancement of Science

articles

Mutation to herbicide resistance maps within the psbA gene of Anacystis nidulans R2

SS Golden and R Haselkorn

A psbA gene encoding the target of photosystem II herbicide inhibition, the 32,000-dalton thylakoid membrane protein, has been cloned from a mutant of Anacystis nidulans R2, which is resistant to 3-(3,4-dichlorophenyl)-1,1-dimethylurea-(diuron). A cloned DNA fragment from within the coding region of this gene transforms wild-type cells to herbicide resistance, proving that mutation within psbA is responsible for that phenotype. The mutation consists of a single nucleotide change that replaces serine at position 264 of the wild-type protein with alanine in that of the diuron-resistant mutant.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Novel psbA1 Gene from a Naturally Occurring Atrazine-Resistant Cyanobacterial Isolate.
K. Sajjaphan, N. Shapir, A. K. Judd, L. P. Wackett, and M. J. Sadowsky (2002)
Appl. Envir. Microbiol. 68, 1358-1366
   Abstract »    Full Text »    PDF »
Genome rearrangement and nitrogen fixation in Anabaena blocked by inactivation of xisA gene.
J. Golden and D. Wiest (1988)
Science 242, 1421-1423
   Abstract »    PDF »


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