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Science 13 September 1985:
Vol. 229. no. 4718, pp. 1102 - 1104
DOI: 10.1126/science.2994213
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Articles

Science, Vol 229, Issue 4718, 1102-1104
Copyright © 1985 by American Association for the Advancement of Science

articles

Contribution of promoter to tissue-specific expression of the mouse immunoglobulin kappa gene

TV Gopal, T Shimada, AW Baur, and AW Nienhuis

The immunoglobulin kappa (kappa) gene promoter was activated by a "neutral" enhancer derived from Harvey murine sarcoma virus (HaMuSV) in immunoglobulin-producing myeloma cells, regardless of the enhancer's orientation or position in the vector. In one fibroblast line (3T3) the immunoglobulin kappa gene promoter was completely inactive when linked to the HaMuSV enhancer, whereas in mouse L cells, promoter activity was observed only with the HaMuSV enhancer in tandem with the immunoglobulin kappa gene promoter. The differential behavior of the gene promoter, when activated by a neutral enhancer in these three murine cell lines, suggests that promoter sequences contribute to the tissue-specific expression of this gene.


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