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Science 6 September 1985:
Vol. 229. no. 4717, pp. 984 - 986
DOI: 10.1126/science.4023718
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Articles

Science, Vol 229, Issue 4717, 984-986
Copyright © 1985 by American Association for the Advancement of Science

articles

Growth regulation of human melanocytes: mitogenic factors in extracts of melanoma, astrocytoma, and fibroblast cell lines

M Eisinger, O Marko, S Ogata, and LJ Old

Melanocytes derived from fetal or adult skin do not propagate in vitro unless cultured in the presence of factors such as 12-O-tetradecanoylphorbol 13-acetate (TPA). In a search for physiological factors regulating the growth of melanocytes, extracts of various cultured cell types were tested. Factors produced by melanoma and astrocytoma cell lines support continued proliferation of melanocytes in the absence of TPA. WI-38, a fibroblast cell line derived from human embryonic lung, was the most active source of melanocyte growth factors. No melanocyte growth-promoting activity was found in extracts of cultured neuroblastoma, renal cancer, normal keratinocytes, or renal epithelium. Nerve growth factor, epidermal growth factor, melanocyte-stimulating hormone, transforming growth factor-beta, and platelet-derived growth factor did not have growth-promoting activity for melanocytes. The presence of melanocyte growth factors and TPA together resulted in the strongest mitogenic activity for melanocytes, permitting the recovery (at 20 days) of 4 to 20 times as many cells as in growth factor or TPA alone.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Different Chromatin Organization in Benign and Malignant Cells Revealed by Unequal Nuclease Sensitivity between Tumor and Normal Cell Genomes.
M. Rieber, M. Strasberg-Rieber, A. J. Maniotis, and R. Folberg (2007)
Am. J. Pathol. 170, 787-789
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Melanin Pigmentation in Mammalian Skin and Its Hormonal Regulation.
A. Slominski, D. J. Tobin, S. Shibahara, and J. Wortsman (2004)
Physiol Rev 84, 1155-1228
   Abstract »    Full Text »    PDF »
Alpha-melanocyte stimulating hormone and its analogue Nle4DPhe7 alpha-MSH affect morphology, tyrosinase activity and melanogenesis in cultured human melanocytes.
G Hunt, C Todd, J. Cresswell, and A. Thody (1994)
J. Cell Sci. 107, 205-211
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