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Science 6 September 1985:
Vol. 229. no. 4717, pp. 978 - 981
DOI: 10.1126/science.2992091
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Articles

Science, Vol 229, Issue 4717, 978-981
Copyright © 1985 by American Association for the Advancement of Science

articles

Isolation and propagation of a human enteric coronavirus

S Resta, JP Luby, CR Rosenfeld, and JD Siegel

Coronavirus-like particles were found by electron microscopy in stools from infants with necrotizing enterocolitis. Stool samples from these infants as well as control specimens were passaged in cultures of human fetal intestinal organs. Two samples yielded virus-like particles and these have now been passaged 14 times (HEC 14). Gradient-purified HEC 14 strains had typical coronavirus morphology on electron microscopy and contained five major proteins with molecular sizes ranging from 190 to 23 kilodaltons. Infants with necrotizing enterocolitis developed specific antibody to the viral antigens between the acute and convalescent stages of the disease, as shown by examining serum specimens by single radial hemolysis, immunoenzymatic assay, and Western immunoblotting. No cross-reactivity was shown with other coronavirus strains tested, or with the newly isolated viruses of the Breda-Berne group, responsible for calf or horse diarrhea.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Coronavirus Pathogenesis and the Emerging Pathogen Severe Acute Respiratory Syndrome Coronavirus.
S. R. Weiss and S. Navas-Martin (2005)
Microbiol. Mol. Biol. Rev. 69, 635-664
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Development of One-Step, Real-Time, Quantitative Reverse Transcriptase PCR Assays for Absolute Quantitation of Human Coronaviruses OC43 and 229E.
L. Vijgen, E. Keyaerts, E. Moes, P. Maes, G. Duson, and M. Van Ranst (2005)
J. Clin. Microbiol. 43, 5452-5456
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Infants Born to Mothers With Severe Acute Respiratory Syndrome.
C. C. Shek, P. C. Ng, G. P. G. Fung, F. W. T. Cheng, P. K. S. Chan, M. J. S. Peiris, K. H. Lee, S. F. Wong, H. M. Cheung, A. M. Li, et al. (2003)
Pediatrics 112, e254-254
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Diagnosis of Noncultivatable Gastroenteritis Viruses, the Human Caliciviruses.
R. L. Atmar and M. K. Estes (2001)
Clin. Microbiol. Rev. 14, 15-37
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Persistent Infection of Human Oligodendrocytic and Neuroglial Cell Lines by Human Coronavirus 229E.
N. Arbour, S. Ekandé, G. Côté, C. Lachance, F. Chagnon, M. Tardieu, N. R. Cashman, and P. J. Talbot (1999)
J. Virol. 73, 3326-3337
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Acute and Persistent Infection of Human Neural Cell Lines by Human Coronavirus OC43.
N. Arbour, G. Côté, C. Lachance, M. Tardieu, N. R. Cashman, and P. J. Talbot (1999)
J. Virol. 73, 3338-3350
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Science. ISSN 0036-8075 (print), 1095-9203 (online)