Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 6 September 1985:
Vol. 229. no. 4717, pp. 932 - 940
DOI: 10.1126/science.2410982
Prev | Table of Contents | Next

Articles

Science, Vol 229, Issue 4717, 932-940
Copyright © 1985 by American Association for the Advancement of Science

articles

Intrinsic and extrinsic factors in protein antigenic structure

JA Berzofsky

Recent advances in the preparation of synthetic peptide vaccines and the use of synthetic peptides as probes of antigenic structure and function have led to renewed interest in the prediction of antigenic sites recognized by antibodies and T cells. This review focuses on antibodies. Features intrinsic to the antigen, such as hydrophilicity and mobility, may be useful in the selection of amino acid sequences of the native protein that will elicit antibodies cross-reacting with peptides, or sequences which, as peptides, will be more likely to elicit antibodies cross-reactive with the native protein. Structural mobility may also contribute to protein-protein interactions in general. However, the entire accessible surface of a protein is likely to be detectable by a large enough panel of antibodies. Which of these antibodies are made in any individual depends on factors extrinsic to the antigen molecule, host factors such as self-tolerance, immune response genes, idiotype networks, and the immunoglobulin structural gene repertoire.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Characterization of the B Cell Epitopes Associated with a Truncated Form of Pseudomonas Exotoxin (PE38) Used to Make Immunotoxins for the Treatment of Cancer Patients.
M. Onda, S. Nagata, D. J. FitzGerald, R. Beers, R. J. Fisher, J. J. Vincent, B. Lee, M. Nakamura, J. Hwang, R. J. Kreitman, et al. (2006)
J. Immunol. 177, 8822-8834
   Abstract »    Full Text »    PDF »
Antibody evolution constrains conformational heterogeneity by tailoring protein dynamics.
J. Zimmermann, E. L. Oakman, I. F. Thorpe, X. Shi, P. Abbyad, C. L. Brooks III, S. G. Boxer, and F. E. Romesberg (2006)
PNAS 103, 13722-13727
   Abstract »    Full Text »    PDF »
Cloning, Characterization, and Serodiagnostic Evaluation of Leishmania infantum Tandem Repeat Proteins.
Y. Goto, R. N. Coler, J. Guderian, R. Mohamath, and S. G. Reed (2006)
Infect. Immun. 74, 3939-3945
   Abstract »    Full Text »    PDF »
Reduction of the inhibitory antibody response to human factor VIII in hemophilia A mice by mutagenesis of the A2 domain B-cell epitope.
E. T. Parker, J. F. Healey, R. T. Barrow, H. N. Craddock, and P. Lollar (2004)
Blood 104, 704-710
   Abstract »    Full Text »    PDF »
Protein dynamics and the immunological evolution of molecular recognition.
R. Jimenez, G. Salazar, J. Yin, T. Joo, and F. E. Romesberg (2004)
PNAS 101, 3803-3808
   Abstract »    Full Text »    PDF »
Identification of Critical Residues of an Immunodominant Region of Echinococcus granulosus Antigen B.
G. Gonzalez-Sapienza and R. E. Cachau (2003)
J. Biol. Chem. 278, 20179-20184
   Abstract »    Full Text »    PDF »
Molecular and structural analysis of the panallergen profilin B cell epitopes defined by monoclonal antibodies.
J. A. Asturias, N. Gomez-Bayon, M. C. Arilla, L. Sanchez-Pulido, A. Valencia, and A. Martinez (2002)
Int. Immunol. 14, 993-1001
   Abstract »    Full Text »    PDF »
Epitope Recognition by Diverse Antibodies Suggests Conformational Convergence in an Antibody Response.
D. T. Nair, K. Singh, Z. Siddiqui, B. P. Nayak, K. V. S. Rao, and D. M. Salunke (2002)
J. Immunol. 168, 2371-2382
   Abstract »    Full Text »    PDF »
Structure of a factor VIII C2 domain-immunoglobulin G4{kappa} Fab complex: identification of an inhibitory antibody epitope on the surface of factor VIII.
P. C. Spiegel Jr, M. Jacquemin, J.-M. R. Saint-Remy, B. L. Stoddard, and K. P. Pratt (2001)
Blood 98, 13-19
   Abstract »    Full Text »    PDF »
B Cell Responses to a Peptide Epitope. X. Epitope Selection in a Primary Response Is Thermodynamically Regulated.
P. Nakra, V. Manivel, R. A. Vishwakarma, and K. V. S. Rao (2000)
J. Immunol. 164, 5615-5625
   Abstract »    Full Text »    PDF »
Dengue Virus Structural Differences That Correlate with Pathogenesis.
K. C. Leitmeyer, D. W. Vaughn, D. M. Watts, R. Salas, I. Villalobos, de Chacon, C. Ramos, and R. Rico-Hesse (1999)
J. Virol. 73, 4738-4747
   Abstract »    Full Text »
B Cell Responses to a Peptide Epitope. V. Kinetic Regulation of Repertoire Discrimination and Antibody Optimization for Epitope.
B. P. Nayak, R. Tuteja, V. Manivel, R. P. Roy, R. A. Vishwakarma, and K. V. S. Rao (1998)
J. Immunol. 161, 3510-3519
   Abstract »    Full Text »    PDF »
Cryptic Antigenic Determinants on the Extracellular Pyruvate Dehydrogenase Complex/Mimeotope Found in Primary Biliary Cirrhosis. A PROBE BY AFFINITY MASS SPECTROMETRY.
T.-T. Yip, J. Van de Water, M. E. Gershwin, R. L. Coppel, and T. W. Hutchens (1996)
J. Biol. Chem. 271, 32825-32833
   Abstract »    Full Text »    PDF »
Molecular and Structural Analysis of a Continuous Birch Profilin Epitope Defined by a Monoclonal Antibody.
P. Wiedemann, K. Giehl, S. C. Almo, A. A. Fedorov, M. Girvin, P. Steinberger, M. Rudiger, M. Ortner, M. Sippl, C. Dolecek, et al. (1996)
J. Biol. Chem. 271, 29915-29921
   Abstract »    Full Text »    PDF »
Vaccination with a synthetic zona pellucida peptide produces long-term contraception in female mice.
S. Millar, S. Chamow, A. Baur, C Oliver, F Robey, and J Dean (1989)
Science 246, 935-938
   Abstract »    PDF »
Development and testing of AIDS vaccines.
W. Koff and D. Hoth (1988)
Science 241, 426-432
   Abstract »    PDF »
Computers in molecular biology: current applications and emerging trends.
C DeLisi (1988)
Science 240, 47-52
   Abstract »    PDF »
Chemistry of antibody binding to a protein.
H. Geysen, J. Tainer, S. Rodda, T. Mason, H Alexander, E. Getzoff, and R. Lerner (1987)
Science 235, 1184-1190
   Abstract »    PDF »
Mechanisms of antibody binding to a protein.
E. Getzoff, H. Geysen, S. Rodda, H Alexander, J. Tainer, and R. Lerner (1987)
Science 235, 1191-1196
   Abstract »    PDF »
Epitope mapping by chemical modification of free and antibody-bound protein antigen.
A Burnens, S Demotz, G Corradin, H Binz, and H. Bosshard (1987)
Science 235, 780-783
   Abstract »    PDF »
Three-dimensional structure of an antigen-antibody complex at 2.8 A resolution.
A. Amit, R. Mariuzza, S. Phillips, and R. Poljak (1986)
Science 233, 747-753
   Abstract »    PDF »
A poliovirus neutralization epitope expressed on hybrid hepatitis B surface antigen particles.
F Delpeyroux, N Chenciner, A Lim, Y Malpiece, B Blondel, R Crainic, S van der Werf, and R. Streeck (1986)
Science 233, 472-475
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)