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Science 23 August 1985:
Vol. 229. no. 4715, pp. 726 - 733
DOI: 10.1126/science.4023707
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Articles

Science, Vol 229, Issue 4715, 726-733
Copyright © 1985 by American Association for the Advancement of Science

articles

Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution

CM Rice, EM Lenches, Eddy SR, SJ Shin, RL Sheets, and JH Strauss

The sequence of the entire RNA genome of the type flavivirus, yellow fever virus, has been obtained. Inspection of this sequence reveals a single long open reading frame of 10,233 nucleotides, which could encode a polypeptide of 3411 amino acids. The structural proteins are found within the amino-terminal 780 residues of this polyprotein; the remainder of the open reading frame consists of nonstructural viral polypeptides. This genome organization implies that mature viral proteins are produced by posttranslational cleavage of a polyprotein precursor and has implications for flavivirus RNA replication and for the evolutionary relation of this virus family to other RNA viruses.


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Mutagenesis of the Signal Sequence of Yellow Fever Virus prM Protein: Enhancement of Signalase Cleavage In Vitro Is Lethal for Virus Production.
E. Lee, C. E. Stocks, S. M. Amberg, C. M. Rice, and M. Lobigs (2000)
J. Virol. 74, 24-32
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Origin of the West Nile Virus Responsible for an Outbreak of Encephalitis in the Northeastern United States.
R. S. Lanciotti, J. T. Roehrig, V. Deubel, J. Smith, M. Parker, K. Steele, B. Crise, K. E. Volpe, M. B. Crabtree, J. H. Scherret, et al. (1999)
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A Novel in Vitro Replication System for Dengue Virus. INITIATION OF RNA SYNTHESIS AT THE 3'-END OF EXOGENOUS VIRAL RNA TEMPLATES REQUIRES 5'- AND 3'-TERMINAL COMPLEMENTARY SEQUENCE MOTIFS OF THE VIRAL RNA.
S. You and R. Padmanabhan (1999)
J. Biol. Chem. 274, 33714-33722
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Markers for trans-Golgi Membranes and the Intermediate Compartment Localize to Induced Membranes with Distinct Replication Functions in Flavivirus-Infected Cells.
J. M. Mackenzie, M. K. Jones, and E. G. Westaway (1999)
J. Virol. 73, 9555-9567
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Genetic Interaction of Flavivirus Nonstructural Proteins NS1 and NS4A as a Determinant of Replicase Function.
B. D. Lindenbach and C. M. Rice (1999)
J. Virol. 73, 4611-4621
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Analysis of Murine CD8+ T-Cell Clones Specific for the Dengue Virus NS3 Protein: Flavivirus Cross-Reactivity and Influence of Infecting Serotype.
A. C. Spaulding, I. Kurane, F. A. Ennis, and A. L. Rothman (1999)
J. Virol. 73, 398-403
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trans-Complementation of Flavivirus RNA Polymerase Gene NS5 by Using Kunjin Virus Replicon-Expressing BHK Cells.
A. A. Khromykh, M. T. Kenney, and E. G. Westaway (1998)
J. Virol. 72, 7270-7279
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Identification of Specific Nucleotide Sequences within the Conserved 3'-SL in the Dengue Type 2 Virus Genome Required for Replication.
L. Zeng, B. Falgout, and L. Markoff (1998)
J. Virol. 72, 7510-7522
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Signal Peptidase Cleavage at the Flavivirus C-prM Junction: Dependence on the Viral NS2B-3 Protease for Efficient Processing Requires Determinants in C, the Signal Peptide, and prM.
C. E. Stocks and M. Lobigs (1998)
J. Virol. 72, 2141-2149
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Mutagenesis of the NS3 Protease of Dengue Virus Type 2.
R. P. C. Valle and B. Falgout (1998)
J. Virol. 72, 624-632
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Association between NS3 and NS5 Proteins of Dengue Virus Type 2 in the Putative RNA Replicase Is Linked to Differential Phosphorylation of NS5.
M. Kapoor, L. Zhang, M. Ramachandra, J. Kusukawa, K. E. Ebner, and R. Padmanabhan (1995)
J. Biol. Chem. 270, 19100-19106
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Insulin-resistant diabetes due to a point mutation that prevents insulin proreceptor processing.
Y Yoshimasa, S Seino, J Whittaker, T Kakehi, A Kosaki, H Kuzuya, H Imura, G. Bell, and D. Steiner (1988)
Science 240, 784-787
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Pathogenesis of dengue: challenges to molecular biology.
S. Halstead (1988)
Science 239, 476-481
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In Vitro RNA Synthesis from Exogenous Dengue Viral RNA Templates Requires Long Range Interactions between 5'- and 3'-Terminal Regions That Influence RNA Structure.
S. You, B. Falgout, L. Markoff, and R. Padmanabhan (2001)
J. Biol. Chem. 276, 15581-15591
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