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Science 12 July 1985:
Vol. 229. no. 4709, pp. 185 - 187
DOI: 10.1126/science.3160112
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Articles

Science, Vol 229, Issue 4709, 185-187
Copyright © 1985 by American Association for the Advancement of Science

articles

Staphylococcal enterotoxin A is encoded by phage

MJ Betley and JJ Mekalanos

The gene for staphylococcal enterotoxin A (entA), in two wild-type strains, is carried by related temperate bacteriophages. Hybridization analysis of DNA from entA-converting phage PS42-D and its bacterial host suggests that this phage integrates into the bacterial chromosome by circularization and reciprocal crossover (the Campbell model) and that the entA gene is located near the phage attachment site. DNA from three of eight staphylococcal strains that did not produce enterotoxin A and seven wild-type enterotoxin A-producing (EntA+) strains had extensive homology to the entA-converting phage PS42-D DNA, although there was a high degree of restriction-fragment length polymorphisms. At least one EntA+ strain did not produce detectable viable phage after induction. These data indicate that a polymorphic family of Staphylococcus aureus phages (some of which may be defective) can carry the entA gene.


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