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Science 5 July 1985:
Vol. 229. no. 4708, pp. 23 - 28
DOI: 10.1126/science.3892686
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Articles

Science, Vol 229, Issue 4708, 23-28
Copyright © 1985 by American Association for the Advancement of Science

articles

Aromatic-aromatic interaction: a mechanism of protein structure stabilization

SK Burley and GA Petsko

Analysis of neighboring aromatic groups in four biphenyl peptides or peptide analogs and 34 proteins reveals a specific aromatic-aromatic interaction. Aromatic pairs (less than 7 A between phenyl ring centroids) were analyzed for the frequency of pair type, their interaction geometry (separation and dihedral angle), their nonbonded interaction energy, the secondary structural locations of interacting residues, their environment, and their conservation in related molecules. The results indicate that on average about 60 percent of aromatic side chains in proteins are involved in aromatic pairs, 80 percent of which form networks of three or more interacting aromatic side chains. Phenyl ring centroids are separated by a preferential distance of between 4.5 and 7 A, and dihedral angles approaching 90 degrees are most common. Nonbonded potential energy calculations indicate that a typical aromatic-aromatic interaction has energy of between -1 and -2 kilocalories per mole. The free energy contribution of the interaction depends on the environment of the aromatic pair. Buried or partially buried pairs constitute 80 percent of the surveyed sample and contribute a free energy of between -0.6 and -1.3 kilocalories per mole to the stability of the protein's structure at physiologic temperature. Of the proteins surveyed, 80 percent of these energetically favorable interactions stabilize tertiary structure, and 20 percent stabilize quaternary structure. Conservation of the interaction in related molecules is particularly striking.


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   Abstract »    PDF »
Three-dimensional structure of an oncogene protein: catalytic domain of human c-H-ras p21.
A. de Vos, L Tong, M. Milburn, P. Matias, J Jancarik, S Noguchi, S Nishimura, K Miura, E Ohtsuka, and S. Kim (1988)
Science 239, 888-893
   Abstract »    PDF »
The C-peptide Helix from Ribonuclease A Considered as an Autonomous Folding Unit.
K.R. Shoemaker, R. Fairman, P.S. Kim, E.J. York, J.M. Stewart, and R.L. Baldwin (1987)
Cold Spring Harb Symp Quant Biol 52, 391-398
   Abstract »    PDF »
Internal Packing and Protein Structural Classes.
J.W. Ponder and F.M. Richards (1987)
Cold Spring Harb Symp Quant Biol 52, 421-428
   Abstract »    PDF »
Structure of pressinoic acid: the cyclic moiety of vasopressin.
D. Langs, G. Smith, J. Stezowski, and R. Hughes (1986)
Science 232, 1240-1242
   Abstract »    PDF »
Design, Engineering, and Production of Human Recombinant T Cell Receptor Ligands Derived from Human Leukocyte Antigen DR2.
J. W. Chang, D. E. Mechling, H.-P. Bachinger, and G. G. Burrows (2001)
J. Biol. Chem. 276, 24170-24176
   Abstract »    Full Text »    PDF »
Analysis of structure and function relationships of an autoantigenic peptide of insulin bound to H-2Kd that stimulates CD8 T cells in insulin-dependent diabetes mellitus.
F. S. Wong, A. K. Moustakas, L. Wen, G. K. Papadopoulos, and C. A. Janeway Jr. (2002)
PNAS 99, 5551-5556
   Abstract »    Full Text »    PDF »


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