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Science 21 June 1985:
Vol. 228. no. 4706, pp. 1443 - 1446
DOI: 10.1126/science.4012301
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Articles

Science, Vol 228, Issue 4706, 1443-1446
Copyright © 1985 by American Association for the Advancement of Science

articles

Heat shock genes: regulatory role for differentiation in parasitic protozoa

LH Van der Ploeg, SH Giannini, and CR Cantor

The parasitic protozoa Trypanosoma brucei and Leishmania major are transmitted by insect vectors to their mammalian hosts. The temperature difference between the hosts (25 degrees and 37 degrees C) may induce a heat shock response in the parasite. Transcripts of heat shock genes (homologous to Hsp70 and Hsp83) were 25 to 100 times more abundant in Trypanosoma brucei bloodstream forms (trypomastigotes) than in insect (procyclic) stages. In Leishmania major the patterns of heat shock gene expression in promastigotes (insect-adapted) and amastigotes (mammal-adapted) were different. A temperature shift in vitro induced differentiation of Leishmania major from promastigotes to amastigotes. Therefore, heat shock genes may be responsible for differentiation of these vector-borne parasites.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Expression of Toxoplasma gondii-Specific Heat Shock Protein 70 during In Vivo Conversion of Bradyzoites to Tachyzoites.
N. M. Silva, R. T. Gazzinelli, D. A. O. Silva, E. A. V. Ferro, L. H. Kasper, and J. R. Mineo (1998)
Infect. Immun. 66, 3959-3963
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Leishmania donovani Heat Shock Protein 100. CHARACTERIZATION AND FUNCTION IN AMASTIGOTE STAGE DIFFERENTIATION.
S. Krobitsch, S. Brandau, C. Hoyer, C. Schmetz, A. Hubel, and J. Clos (1998)
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Cell Wall and Secreted Proteins of Candida albicans: Identification, Function, and Expression.
W. L. Chaffin, J. L. Lopez-Ribot, M. Casanova, D. Gozalbo, and J. P. Martinez (1998)
Microbiol. Mol. Biol. Rev. 62, 130-180
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Analysis of Post-transcriptional Regulation Operating on Transcription Products of the Tandemly Linked Leishmania infantum hsp70 Genes.
L. Quijada, M. Soto, C. Alonso, and J. M. Requena (1997)
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Modulation of protein synthesis relative to DNA synthesis alters the timing of differentiation in the protozoan parasite Theileria annulata.
B Shiels, N Aslam, S McKellar, A Smyth, and J Kinnaird (1997)
J. Cell Sci. 110, 1441-1451
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Expression of a mitochondrial stress protein in the protozoan parasite Leishmania major.
S Searle, M. McCrossan, and D. Smith (1993)
J. Cell Sci. 104, 1091-1100
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Karyotype analysis of Leishmania species and its use in classification and clinical diagnosis.
S. Giannini, M Schittini, J. Keithly, P. Warburton, C. Cantor, and L. Van der Ploeg (1986)
Science 232, 762-765
   Abstract »    PDF »


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