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Science 14 June 1985:
Vol. 228. no. 4705, pp. 1329 - 1331
DOI: 10.1126/science.4001946
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Articles

Science, Vol 228, Issue 4705, 1329-1331
Copyright © 1985 by American Association for the Advancement of Science

articles

Monitoring the time course of cerebral deoxyglucose metabolism by 31P nuclear magnetic resonance spectroscopy

RK Deuel, GM Yue, WR Sherman, DJ Schickner, and JJ Ackerman

The phosphorylation of 2-deoxyglucose by the mammalian brain is used as an index of the brain's energy metabolism. The results of phosphorus-31 nuclear magnetic resonance (31P NMR) monitoring of conscious animals in vivo showed rapid phosphorylation of 2-deoxyglucose by brain tissue. The rate of phosphorylation as determined by 31P NMR was consistent with results achieved by tracer methods using carbon-14-labeled 2-deoxyglucose. However, the disappearance of 2-deoxyglucose-6-phosphate was shown to be faster than that reported by tracer studies and occurred without alterations of intracellular pH and energy homeostasis. These results were confirmed by gas chromatography and mass spectroscopy. It is postulated that 2-deoxyglucose may be metabolized in several ways, including dephosphorylation by a hexose phosphatase.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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D. Rivenzon-Segal, E. Rushkin, S. Polak-Charcon, and H. Degani (2000)
Am J Physiol Endocrinol Metab 279, E508-E519
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Measurement of brain deoxyglucose metabolism by NMR.
T Nelson, G Lucignani, and L Sokoloff (1986)
Science 232, 776-777
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