Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 14 June 1985:
Vol. 228. no. 4705, pp. 1313 - 1315
DOI: 10.1126/science.4001943
Prev | Table of Contents | Next

Articles

Science, Vol 228, Issue 4705, 1313-1315
Copyright © 1985 by American Association for the Advancement of Science

articles

Microinjected c-myc as a competence factor

L Kaczmarek, JK Hyland, R Watt, M Rosenberg, and R Baserga

While a number of oncogenes are expressed in a cell cycle-dependent manner, their role in the control of cell proliferation can only be established by a direct functional assay. The c-myc protein, upon microinjection into nuclei of quiescent Swiss 3T3 cells, cooperated with platelet-poor plasma in the stimulation of cellular DNA synthesis. This suggests that c-myc protein, like platelet-derived growth factor (PDGF), may act as a competence factor in the cell cycle to promote the progression of cells to S phase. The presence in the medium of an antibody against PDGF abolished DNA synthesis induced by microinjected PDGF; however, the microinjected c-myc protein stimulated DNA synthesis even when its own antibody was present in the medium. The c-myc protein may act as an intracellular competence factor, while PDGF expresses its biological activity only from outside the cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Insertional Mutagenesis Reveals Progression Genes and Checkpoints in MYC/Runx2 Lymphomas.
M. Stewart, N. Mackay, L. Hanlon, K. Blyth, L. Scobie, E. Cameron, and J. C. Neil (2007)
Cancer Res. 67, 5126-5133
   Abstract »    Full Text »    PDF »
Distinct Structural Features ofCaprin-1 Mediate Its Interaction with G3BP-1 and Its Induction of Phosphorylation of Eukaryotic Translation Initiation Factor 2{alpha}, Entry to Cytoplasmic Stress Granules, and Selective Interaction with a Subset of mRNAs.
S. Solomon, Y. Xu, B. Wang, M. D. David, P. Schubert, D. Kennedy, and J. W. Schrader (2007)
Mol. Cell. Biol. 27, 2324-2342
   Abstract »    Full Text »    PDF »
Roles for c-Myc in Self-renewal of Hematopoietic Stem Cells.
Y. Satoh, I. Matsumura, H. Tanaka, S. Ezoe, H. Sugahara, M. Mizuki, H. Shibayama, E. Ishiko, J. Ishiko, K. Nakajima, et al. (2004)
J. Biol. Chem. 279, 24986-24993
   Abstract »    Full Text »    PDF »
Loss of Protooncogene c-Myc Function Impedes G1 Phase Progression Both before and after the Restriction Point.
C. Schorl and J. M. Sedivy (2003)
Mol. Biol. Cell 14, 823-835
   Abstract »    Full Text »    PDF »
Guanine Nucleotide Exchange Factor, Tiam1, Directly Binds to c-Myc and Interferes with c-Myc-mediated Apoptosis in Rat-1 Fibroblasts.
Y. Otsuki, M. Tanaka, T. Kamo, C. Kitanaka, Y. Kuchino, and H. Sugimura (2003)
J. Biol. Chem. 278, 5132-5140
   Abstract »    Full Text »    PDF »
The Proto-oncogene c-myc Acts through the Cyclin-dependent Kinase (Cdk) Inhibitor p27Kip1 to Facilitate the Activation of Cdk4/6 and Early G1 Phase Progression.
A. J. Obaya, I. Kotenko, M. D. Cole, and J. M. Sedivy (2002)
J. Biol. Chem. 277, 31263-31269
   Abstract »    Full Text »    PDF »
Inactivation of E2f1 Enhances Tumorigenesis in a Myc Transgenic Model.
R. J. Rounbehler, P. M. Rogers, C. J. Conti, and D. G. Johnson (2002)
Cancer Res. 62, 3276-3281
   Abstract »    Full Text »    PDF »
Mad1 Function Is Regulated through Elements within the Carboxy Terminus.
G. Barrera-Hernandez, C. M. Cultraro, S. Pianetti, and S. Segal (2000)
Mol. Cell. Biol. 20, 4253-4264
   Abstract »    Full Text »
Epidermal Growth Factor and Insulin-Induced Deoxyribonucleic Acid Synthesis in Primary Rat Hepatocytes Is Phosphatidylinositol 3-Kinase Dependent and Dissociated from Protooncogene Induction.
C. J. Band, C. Mounier, and B. I. Posner (1999)
Endocrinology 140, 5626-5634
   Abstract »    Full Text »
c-Myc Regulates Cyclin D-Cdk4 and -Cdk6 Activity but Affects Cell Cycle Progression at Multiple Independent Points.
M. K. Mateyak, A. J. Obaya, and J. M. Sedivy (1999)
Mol. Cell. Biol. 19, 4672-4683
   Abstract »    Full Text »    PDF »
Antisense TR3 Orphan Receptor Can Increase Prostate Cancer Cell Viability with Etoposide Treatment.
H. Uemura and C. Chang (1998)
Endocrinology 139, 2329-2334
   Abstract »    Full Text »    PDF »
Frequent provirus insertional mutagenesis of Notch1 in thymomas of MMTVD/myc transgenic mice suggests a collaboration of c-myc and Notch1 for oncogenesis..
L Girard, Z Hanna, N Beaulieu, C D Hoemann, C Simard, C A Kozak, and P Jolicoeur (1996)
Genes & Dev. 10, 1930-1944
   Abstract »    PDF »
Estrogen Reduces Myointimal Proliferation After Balloon Injury of Rat Carotid Artery.
S.-J. Chen, H. Li, J. Durand, S. Oparil, and Y.-F. Chen (1996)
Circulation 93, 577-584
   Abstract »    Full Text »
c-myc in Vasculoproliferative Disease.
E. R. Edelman, M. Simons, M. G. Sirois, and R. D. Rosenberg (1995)
Circ. Res. 76, 176-182
   Abstract »    Full Text »
Identification of serum-inducible genes: different patterns of gene regulation during G0-->S and G1-->S progression.
M Wick, C Burger, S Brusselbach, F. Lucibello, and R Muller (1994)
J. Cell Sci. 107, 227-239
   Abstract »    PDF »
Inhibition of serum- and ras-stimulated DNA synthesis by antibodies to phospholipase C.
M. Smith, Y. Liu, H Kim, S. Rhee, and H. Kung (1990)
Science 247, 1074-1077
   Abstract »    PDF »
A cell-cycle constraint on the regulation of gene expression by platelet-derived growth factor.
B. Rollins, E. Morrison, and C. Stiles (1987)
Science 238, 1269-1271
   Abstract »    PDF »
Participation of c-myc protein in DNA synthesis of human cells.
G. Studzinski, Z. Brelvi, S. Feldman, and R. Watt (1986)
Science 234, 467-470
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)