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Science 14 June 1985:
Vol. 228. no. 4705, pp. 1313 - 1315
DOI: 10.1126/science.4001943
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Articles

Science, Vol 228, Issue 4705, 1313-1315
Copyright © 1985 by American Association for the Advancement of Science

articles

Microinjected c-myc as a competence factor

L Kaczmarek, JK Hyland, R Watt, M Rosenberg, and R Baserga

While a number of oncogenes are expressed in a cell cycle-dependent manner, their role in the control of cell proliferation can only be established by a direct functional assay. The c-myc protein, upon microinjection into nuclei of quiescent Swiss 3T3 cells, cooperated with platelet-poor plasma in the stimulation of cellular DNA synthesis. This suggests that c-myc protein, like platelet-derived growth factor (PDGF), may act as a competence factor in the cell cycle to promote the progression of cells to S phase. The presence in the medium of an antibody against PDGF abolished DNA synthesis induced by microinjected PDGF; however, the microinjected c-myc protein stimulated DNA synthesis even when its own antibody was present in the medium. The c-myc protein may act as an intracellular competence factor, while PDGF expresses its biological activity only from outside the cells.


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