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Science 24 May 1985:
Vol. 228. no. 4702, pp. 963 - 967
DOI: 10.1126/science.3890181
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Articles

Science, Vol 228, Issue 4702, 963-967
Copyright © 1985 by American Association for the Advancement of Science

articles

The "spliceosome": yeast pre-messenger RNA associates with a 40S complex in a splicing-dependent reaction

E Brody and J Abelson

The in vitro splicing reactions of pre-messenger RNA (pre-mRNA) in a yeast extract were analyzed by glycerol gradient centrifugation. Labeled pre-mRNA appears in a 40S peak only if the pre-mRNA undergoes the first of the two partial splicing reactions. RNA analysis after extraction of glycerol gradient fractions shows that lariat-form intermediates, molecules that occur only in mRNA splicing, are found almost exclusively in this 40S complex. Another reaction intermediate, cut 5' exon RNA, can also be found concentrated in this complex. The complex is stable even in 400 mM KCl, although at this salt concentration, it sediments at 35S and is clearly distinguishable from 40S ribosomal subunits. This complex, termed a "spliceosome," is thought to contain components necessary for mRNA splicing; its existence can explain how separated exons on pre-mRNA are brought into contact.


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U2AF homolog required for splicing in vivo.
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The role of exon sequences in splice site selection..
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An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway..
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In vivo UV cross-linking of U snRNAs that participate in trypanosome trans-splicing..
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RNA secondary structure repression of a muscle-specific exon in HeLa cell nuclear extracts.
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Spliceosomes and snurposomes.
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A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family..
E J Strauss and C Guthrie (1991)
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Control of alternative splicing by the differential binding of U1 small nuclear ribonucleoprotein particle.
H. Kuo, F. Nasim, and P. Grabowski (1991)
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Messenger RNA transport and HIV rev regulation.
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A Sequential splicing mechanism promotes selection of an optimal exon by repositioning a downstream 5' splice site in preprotachykinin pre-mRNA..
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Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae..
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RNA-protein interactions in 30S ribosomal subunits: folding and function of 16S rRNA.
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Genetic analysis of the role of human U1 snRNA in mRNA splicing: I. Effect of mutations in the highly conserved stem-loop I of U1..
C Y Yuo and A M Weiner (1989)
Genes & Dev. 3, 697-707
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RNA Splicing and Genes.
P. A. Sharp (1988)
JAMA 260, 3035-3041
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An early hierarchic role of U1 small nuclear ribonucleoprotein in spliceosome assembly.
S. Ruby and J Abelson (1988)
Science 242, 1028-1035
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Alternative splicing of SV40 early pre-mRNA is determined by branch site selection..
J C Noble, C Prives, and J L Manley (1988)
Genes & Dev. 2, 1460-1475
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Splice site selection, rate of splicing, and alternative splicing on nascent transcripts..
A L Beyer and Y N Osheim (1988)
Genes & Dev. 2, 754-765
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A chemical modification/interference study of yeast pre-mRNA spliceosome assembly and splicing..
B C Rymond and M Rosbash (1988)
Genes & Dev. 2, 428-439
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Spliceosome assembly in yeast..
S C Cheng and J Abelson (1987)
Genes & Dev. 1, 1014-1027
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A mutational analysis of spliceosome assembly: evidence for splice site collaboration during spliceosome formation..
A I Lamond, M M Konarska, and P A Sharp (1987)
Genes & Dev. 1, 532-543
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A trans-acting suppressor restores splicing of a yeast intron with a branch point mutation..
J R Couto, J Tamm, R Parker, and C Guthrie (1987)
Genes & Dev. 1, 445-455
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A novel role for the 3' region of introns in pre-mRNA splicing of Saccharomyces cerevisiae..
B C Rymond, D D Torrey, and M Rosbash (1987)
Genes & Dev. 1, 238-246
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Applying genetics to the splicing problem.
J. R. Warner (1987)
Genes & Dev. 1, 1-3
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Splicing of yeast nuclear pre-mRNA in vitro requires a functional 40S spliceosome and several extrinsic factors..
R J Lin, A J Lustig, and J Abelson (1987)
Genes & Dev. 1, 7-18
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Splicing of messenger RNA precursors.
P. Sharp (1987)
Science 235, 766-771
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Splicing of Messenger RNA Precursors.
P.A. Sharp, M.M. Konarksa, P.J. Grabowski, A.I. Lamond, R. Marciniak, and S.R. Seiler (1987)
Cold Spring Harb Symp Quant Biol 52, 277-285
   Abstract »    PDF »
Different Small Nuclear Ribonucleoprotein Particles Are Involved in Different Steps of Splicing Complex Formation.
D. Frendewey, A. Kramer, and W. Keller (1987)
Cold Spring Harb Symp Quant Biol 52, 287-298
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Affinity chromatography of splicing complexes: U2, U5, and U4 + U6 small nuclear ribonucleoprotein particles in the spliceosome.
P. Grabowski and P. Sharp (1986)
Science 233, 1294-1299
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Heterogeneous nuclear ribonucleoproteins: role in RNA splicing.
Y. Choi, P. Grabowski, P. Sharp, and G Dreyfuss (1986)
Science 231, 1534-1539
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The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein.
B Chabot, D. Black, D. LeMaster, and J. Steitz (1985)
Science 230, 1344-1349
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