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Science 24 May 1985:
Vol. 228. no. 4702, pp. 958 - 962
DOI: 10.1126/science.2988125
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Articles

Science, Vol 228, Issue 4702, 958-962
Copyright © 1985 by American Association for the Advancement of Science

articles

Expression of Plasmodium falciparum circumsporozoite proteins in Escherichia coli for potential use in a human malaria vaccine

JF Young, WT Hockmeyer, M Gross, WR Ballou, RA Wirtz, JH Trosper, RL Beaudoin, MR Hollingdale, LH Miller, CL Diggs, and al. et

The circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum may be the most promising target for the development of a malaria vaccine. In this study, proteins composed of 16, 32, or 48 tandem copies of a tetrapeptide repeating sequence found in the CS protein were efficiently expressed in the bacterium Escherichia coli. When injected into mice, these recombinant products resulted in the production of high titers of antibodies that reacted with the authentic CS protein on live sporozoites and blocked sporozoite invasion of human hepatoma cells in vitro. These CS protein derivatives are therefore candidates for a human malaria vaccine.


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Conserved repetitive epitope recognized by CD4+ clones from a malaria-immunized volunteer.
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Health Advice for International Travel.
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Proteosome-lipopeptide vaccines: enhancement of immunogenicity for malaria CS peptides.
G. Lowell, W. Ballou, L. Smith, R. Wirtz, W. Zollinger, and W. Hockmeyer (1988)
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Oral Salmonella typhimurium vaccine expressing circumsporozoite protein protects against malaria.
J. Sadoff, W. Ballou, L. Baron, W. Majarian, R. Brey, W. Hockmeyer, J. Young, S. Cryz, J Ou, G. Lowell, et al. (1988)
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Naturally acquired antibodies to sporozoites do not prevent malaria: vaccine development implications.
S. Hoffman, C. Oster, C. Plowe, G. Woollett, J. Beier, J. Chulay, R. Wirtz, M. Hollingdale, and M Mugambi (1987)
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Efficacy of murine malaria sporozoite vaccines: implications for human vaccine development.
J. Egan, J. Weber, W. Ballou, M. Hollingdale, W. Majarian, D. Gordon, W. Maloy, S. Hoffman, R. Wirtz, I Schneider, et al. (1987)
Science 236, 453-456
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Construction of synthetic immunogen: use of new T-helper epitope on malaria circumsporozoite protein.
M. Good, W. Maloy, M. Lunde, H Margalit, J. Cornette, G. Smith, B Moss, L. Miller, and J. Berzofsky (1987)
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Research toward malaria vaccines.
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Effect of antibodies to recombinant and synthetic peptides on P. falciparum sporozoites in vitro.
D Mazier, S Mellouk, R. Beaudoin, B Texier, P Druilhe, W Hockmeyer, J Trosper, C Paul, Y Charoenvit, J Young, et al. (1986)
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Sequence of the immunodominant epitope for the surface protein on sporozoites of Plasmodium vivax.
T. McCutchan, A. Lal, V. de la Cruz, L. Miller, W. Maloy, Y Charoenvit, R. Beaudoin, P Guerry, R Wistar Jr, S. Hoffman, et al. (1985)
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Immunogenicity of synthetic peptides from circumsporozoite protein of Plasmodium falciparum.
W. Ballou, J Rothbard, R. Wirtz, D. Gordon, J. Williams, R. Gore, I Schneider, M. Hollingdale, R. Beaudoin, W. Maloy, et al. (1985)
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