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Science 17 May 1985:
Vol. 228. no. 4701, pp. 810 - 815
DOI: 10.1126/science.3923623
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Articles

Science, Vol 228, Issue 4701, 810-815
Copyright © 1985 by American Association for the Advancement of Science

articles

Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins

GG Wong, JS Witek, PA Temple, KM Wilkens, AC Leary, DP Luxenberg, SS Jones, EL Brown, RM Kay, EC Orr, and al. et

Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce recombinant GM-CSF in mammalian cells. The recombinant hematopoietin was similar to the natural product that was purified to apparent homogeneity from medium conditioned by a human T-cell line. The human T-cell GM-CSF was found to be 60 percent homologous with the GM-CSF recently cloned from murine lung messenger RNA.


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