Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 10 May 1985:
Vol. 228. no. 4700, pp. 677 - 685
DOI: 10.1126/science.3158075
Prev | Table of Contents | Next

Articles

Science, Vol 228, Issue 4700, 677-685
Copyright © 1985 by American Association for the Advancement of Science

articles

DNA elements are asymmetrically joined during the site-specific recombination of kappa immunoglobulin genes

S Lewis, A Gifford, and D Baltimore

Immunoglobulin K genes are constructed during lymphocyte differentiation by the joining of two DNA elements, VK and JK, to form both a VKJK coding unit and a reciprocal recombination product. The two products formed in single VK-to-JK joining events can be directly isolated through the use of a retrovirally introduced recombination substrate. The structural analysis of a number of recombinants and the derivation of secondary recombination products define some of the basic features of the mechanism of immunoglobulin gene assembly.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The NF-{kappa}B Canonical Pathway Is Involved in the Control of the Exonucleolytic Processing of Coding Ends during V(D)J Recombination.
M. M. Souto-Carneiro, R. Fritsch, N. Sepulveda, M. J. Lagareiro, N. Morgado, N. S. Longo, and P. E. Lipsky (2008)
J. Immunol. 180, 1040-1049
   Abstract »    Full Text »    PDF »
Diverse Immunoglobulin Light Chain Organizations in Fish Retain Potential to Revise B Cell Receptor Specificities.
E. Hsu and M. F. Criscitiello (2006)
J. Immunol. 177, 2452-2462
   Abstract »    Full Text »    PDF »
Genomic instability due to V(D)J recombination-associated transposition..
Y. V.R. Reddy, E. J. Perkins, and D. A. Ramsden (2006)
Genes & Dev. 20, 1575-1582
   Abstract »    Full Text »    PDF »
Unraveling the Consecutive Recombination Events in the Human IGK Locus.
A. W. Langerak, B. Nadel, A. de Torbal, I. L. M. Wolvers-Tettero, E. J. van Gastel-Mol, B. Verhaaf, U. Jager, and J. J. M. van Dongen (2004)
J. Immunol. 173, 3878-3888
   Abstract »    Full Text »    PDF »
Analyses of TCRB Rearrangements Substantiate a Profound Deficit in Recombination Signal Sequence Joining in SCID Foals: Implications for the Role of DNA-Dependent Protein Kinase in V(D)J Recombination.
E. K. Shin, T. Rijkers, A. Pastink, and K. Meek (2000)
J. Immunol. 164, 1416-1424
   Abstract »    Full Text »    PDF »
Onset and Dynamics of Osteosclerosis in Mice Induced by Reilly-Finkel-Biskis (RFB) Murine Leukemia Virus : Increase in Bone Mass Precedes Lymphomagenesis.
J. Schmidt, K. Lumniczky, B. D. Tzschaschel, H. L. Guenther, A. Luz, S. Riemann, W. Gimbel, V. Erfle, and R. G. Erben (1999)
Am. J. Pathol. 155, 557-570
   Abstract »    Full Text »    PDF »
Modulation of Terminal Deoxynucleotidyltransferase Activity by the DNA-Dependent Protein Kinase.
S. Mickelsen, C. Snyder, K. Trujillo, M. Bogue, D. B. Roth, and K. Meek (1999)
J. Immunol. 163, 834-843
   Abstract »    Full Text »    PDF »
Extensive Junctional Diversity in Ig Light Chain Genes from Early B Cell Progenitors of {micro}MT Mice.
L. A. Bentolila, S. Olson, A. Marshall, F. Rougeon, C. J. Paige, N. Doyen, and G. E. Wu (1999)
J. Immunol. 162, 2123-2128
   Abstract »    Full Text »    PDF »
Extensive Junctional Diversity of Ig Heavy Chain Rearrangements Generated in the Progeny of Single Fetal Multipotent Hematopoietic Cells in the Absence of Selection.
F. Nourrit, N. Doyen, P. Kourilsky, F. Rougeon, and A. Cumano (1998)
J. Immunol. 160, 4254-4261
   Abstract »    Full Text »    PDF »
Formation and resolution of double-strand break intermediates in V(D)J rearrangement..
D A Ramsden and M Gellert (1995)
Genes & Dev. 9, 2409-2420
   Abstract »    PDF »
Double-strand signal sequence breaks in V(D)J recombination are blunt, 5'-phosphorylated, RAG-dependent, and cell cycle regulated..
M Schlissel, A Constantinescu, T Morrow, M Baxter, and A Peng (1993)
Genes & Dev. 7, 2520-2532
   Abstract »    PDF »
Analysis of junctional diversity during B lymphocyte development.
K Meek (1990)
Science 250, 820-823
   Abstract »    PDF »
NBP, a protein that specifically binds an enhancer of immunoglobulin gene rearrangement: purification and characterization..
M Li, E Morzycka-Wroblewska, and S V Desiderio (1989)
Genes & Dev. 3, 1801-1813
   Abstract »    PDF »
V(D)J recombination: a functional definition of the joining signals..
J E Hesse, M R Lieber, K Mizuuchi, and M Gellert (1989)
Genes & Dev. 3, 1053-1061
   Abstract »    PDF »
Unusual immunoglobulin gene rearrangement leads to replacement of recombinational signal sequences.
E Morzycka-Wroblewska, F. Lee, and S. Desiderio (1988)
Science 242, 261-263
   Abstract »    PDF »
The scid gene encodes a trans-acting factor that mediates the rejoining event of Ig gene rearrangement..
E A Hendrickson, D G Schatz, and D T Weaver (1988)
Genes & Dev. 2, 817-829
   Abstract »    PDF »
Development of the primary antibody repertoire.
F. Alt, T. Blackwell, and G. Yancopoulos (1987)
Science 238, 1079-1087
   Abstract »    PDF »
Two pairs of recombination signals are sufficient to cause immunoglobulin V-(D)-J joining.
S Akira, K Okazaki, and H Sakano (1987)
Science 238, 1134-1138
   Abstract »    PDF »
Developmental stage specificity of the lymphoid V(D)J recombination activity..
M R Lieber, J E Hesse, K Mizuuchi, and M Gellert (1987)
Genes & Dev. 1, 751-761
   Abstract »    PDF »
Endonucleolytic activity that cleaves immunoglobulin recombination sequences.
T. Hope, R. Aguilera, M. Minie, and H Sakano (1986)
Science 231, 1141-1145
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)