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Science 3 May 1985:
Vol. 228. no. 4699, pp. 593 - 595
DOI: 10.1126/science.2984774
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Articles

Science, Vol 228, Issue 4699, 593-595
Copyright © 1985 by American Association for the Advancement of Science

articles

Characterization of envelope and core structural gene products of HTLV-III with sera from AIDS patients

WG Robey, B Safai, S Oroszlan, LO Arthur, MA Gonda, RC Gallo, and PJ Fischinger

The envelope (env) and structural (gag) gene products of human T-cell leukemia (lymphotropic) virus type III were identified by immunoaffinity chromatography, immunoprecipitation, and two-dimensional oligopeptide mapping methods. The env gene specifies a glycosylated polypeptide with a molecular weight of 160,000 (gp160) that is processed to gp120 and smaller gene products. The gag gene specifies two polypeptides of 70,000 and 55,000 molecular weight (p70 and p55), both of which contain p24, the major structural protein of the mature virion. The techniques in this study can be used to define the extent of variability of the env gene product among different virus isolates and may identify the nature and patterns of the humoral immune response that lead to an immunologically protected state.


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Reverse transcription of the HIV-1 pandemic.
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Noninfectious entry of HIV-1 into peripheral and brain macrophages mediated by the mannose receptor.
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Characterization of the Multiple Conformational States of Free Monomeric and Trimeric Human Immunodeficiency Virus Envelope Glycoproteins after Fixation by Cross-Linker.
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J. Virol. 80, 6725-6737
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Sequential Immunization with V3 Peptides from Primary Human Immunodeficiency Virus Type 1 Produces Cross-Neutralizing Antibodies against Primary Isolates with a Matching Narrow-Neutralization Sequence Motif..
Y. Eda, M. Takizawa, T. Murakami, H. Maeda, K. Kimachi, H. Yonemura, S. Koyanagi, K. Shiosaki, H. Higuchi, K. Makizumi, et al. (2006)
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HIV-1 Envelope Triggers Polyclonal Ig Class Switch Recombination through a CD40-Independent Mechanism Involving BAFF and C-Type Lectin Receptors.
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J. Immunol. 176, 3931-3941
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Characterization of Antibody Responses Elicited by Human Immunodeficiency Virus Type 1 Primary Isolate Trimeric and Monomeric Envelope Glycoproteins in Selected Adjuvants.
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Characterization of the Outer Domain of the gp120 Glycoprotein from Human Immunodeficiency Virus Type 1.
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J. Virol. 76, 5315-5325
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Highly Stable Trimers Formed by Human Immunodeficiency Virus Type 1 Envelope Glycoproteins Fused with the Trimeric Motif of T4 Bacteriophage Fibritin.
X. Yang, J. Lee, E. M. Mahony, P. D. Kwong, R. Wyatt, and J. Sodroski (2002)
J. Virol. 76, 4634-4642
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Solid-Phase Proteoliposomes Containing Human Immunodeficiency Virus Envelope Glycoproteins.
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J. Virol. 76, 3511-3521
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The Stoichiometry of Trimeric SIV Glycoprotein Interaction with CD4 Differs from That of Anti-envelope Antibody Fab Fragments.
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Envelope Glycoprotein Determinants of Neutralization Resistance in a Simian-Human Immunodeficiency Virus (SHIV-HXBc2P 3.2) Derived by Passage in Monkeys.
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Loss of a Single N-Linked Glycan Allows CD4-Independent Human Immunodeficiency Virus Type 1 Infection by Altering the Position of the gp120 V1/V2 Variable Loops.
P. Kolchinsky, E. Kiprilov, P. Bartley, R. Rubinstein, and J. Sodroski (2001)
J. Virol. 75, 3435-3443
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Increased Neutralization Sensitivity of CD4-Independent Human Immunodeficiency Virus Variants.
P. Kolchinsky, E. Kiprilov, and J. Sodroski (2001)
J. Virol. 75, 2041-2050
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Improved Elicitation of Neutralizing Antibodies against Primary Human Immunodeficiency Viruses by Soluble Stabilized Envelope Glycoprotein Trimers.
X. Yang, R. Wyatt, and J. Sodroski (2001)
J. Virol. 75, 1165-1171
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Characterization of Stable, Soluble Trimers Containing Complete Ectodomains of Human Immunodeficiency Virus Type 1 Envelope Glycoproteins.
X. Yang, M. Farzan, R. Wyatt, and J. Sodroski (2000)
J. Virol. 74, 5716-5725
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Modifications That Stabilize Human Immunodeficiency Virus Envelope Glycoprotein Trimers in Solution.
X. Yang, L. Florin, M. Farzan, P. Kolchinsky, P. D. Kwong, J. Sodroski, and R. Wyatt (2000)
J. Virol. 74, 4746-4754
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Adaptation of a CCR5-Using, Primary Human Immunodeficiency Virus Type 1 Isolate for CD4-Independent Replication.
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J. Virol. 73, 8120-8126
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HIV-1 Antigen-specific and -nonspecific B Cell Responses Are Sensitive to Combination Antiretroviral Therapy.
L. Morris, J. M. Binley, B. A. Clas, S. Bonhoeffer, T. P. Astill, R. Kost, A. Hurley, Y. Cao, M. Markowitz, D. D. Ho, et al. (1998)
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Use of Murine CXCR-4 as a Second Receptor by Some T-Cell-Tropic Human Immunodeficiency Viruses.
C. Parolin, A. Borsetti, H. Choe, M. Farzan, P. Kolchinsky, M. Heesen, Q. Ma, C. Gerard, G. Palu, M. E. Dorf, et al. (1998)
J. Virol. 72, 1652-1656
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Two Orphan Seven-Transmembrane Segment Receptors Which Are Expressed in CD4-positive Cells Support Simian Immunodeficiency Virus Infection.
M. Farzan, H. Choe, K. Martin, L. Marcon, W. Hofmann, G. Karlsson, Y. Sun, P. Barrett, N. Marchand, N. Sullivan, et al. (1997)
J. Exp. Med. 186, 405-411
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Dissociation of gp120 from HIV-1 virions induced by soluble CD4.
J. Moore, J. McKeating, R. Weiss, and Q. Sattentau (1990)
Science 250, 1139-1142
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Prospects for Prevention of and Early Intervention Against HIV.
D. P. Bolognesi (1989)
JAMA 261, 3007-3013
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Unexpectedly high levels of HIV-1 RNA and protein synthesis in a cytocidal infection.
M Somasundaran and H. Robinson (1988)
Science 242, 1554-1557
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Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1.
M Kowalski, J Potz, L Basiripour, T Dorfman, W. Goh, E Terwilliger, A Dayton, C Rosen, W Haseltine, and J Sodroski (1987)
Science 237, 1351-1355
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HTLV-III/LAV-neutralizing antibodies to an E. coli-produced fragment of the virus envelope.
S. Putney, T. Matthews, W. Robey, D. Lynn, M Robert-Guroff, W. Mueller, A. Langlois, J Ghrayeb, S. Petteway Jr, K. Weinhold, et al. (1986)
Science 234, 1392-1395
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Alterations in T4 (CD4) protein and mRNA synthesis in cells infected with HIV.
J. Hoxie, J. Alpers, J. Rackowski, K Huebner, B. Haggarty, A. Cedarbaum, and J. Reed (1986)
Science 234, 1123-1127
   Abstract »    PDF »
A new HTLV-III/LAV protein encoded by a gene found in cytopathic retroviruses.
T. Lee, J. Coligan, J. Allan, M. McLane, J. Groopman, and M Essex (1986)
Science 231, 1546-1549
   Abstract »    PDF »
Replicative and cytopathic potential of HTLV-III/LAV with sor gene deletions.
J Sodroski, W. Goh, C Rosen, A Tartar, D Portetelle, A Burny, and W Haseltine (1986)
Science 231, 1549-1553
   Abstract »    PDF »
Identification of HTLV-III/LAV sor gene product and detection of antibodies in human sera.
N. Kan, G Franchini, F Wong-Staal, G. DuBois, W. Robey, J. Lautenberger, and T. Papas (1986)
Science 231, 1553-1555
   Abstract »    PDF »
Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV.
F di Marzo Veronese, T. Copeland, A. DeVico, R Rahman, S Oroszlan, R. Gallo, and M. Sarngadharan (1986)
Science 231, 1289-1291
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Primary Human T-Lymphotropic Virus Type III Infection.
D. D. HO, M. G. SARNGADHARAN, L. RESNICK, F. DIMARZOVERONESE, T. R. ROTA, and M. S. HIRSCH (1985)
Ann Intern Med 103, 880-883
   Abstract »    PDF »
A new HTLV-III/LAV encoded antigen detected by antibodies from AIDS patients.
J. Allan, J. Coligan, T. Lee, M. McLane, P. Kanki, J. Groopman, and M Essex (1985)
Science 230, 810-813
   Abstract »    PDF »
Antigens of Human T-Lymphotropic Virus Type III/Lymphadenopathy-Associated Virus.
M. ESSEX, J. ALLAN, P. KANKI, M. F. McLANE, G. MALONE, L. KITCHEN, and T. H. LEE (1985)
Ann Intern Med 103, 700-703
   Abstract »    PDF »
Characterization of gp41 as the transmembrane protein coded by the HTLV-III/LAV envelope gene.
F. Veronese, A. DeVico, T. Copeland, S Oroszlan, R. Gallo, and M. Sarngadharan (1985)
Science 229, 1402-1405
   Abstract »    PDF »


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