Science, Vol 228, Issue 4698, 490-492
Copyright © 1985 by American Association for the Advancement of Science
Monoclonal antibody-directed radioimmunoassay detects cytochrome P-450 in human placenta and lymphocytes
BJ Song,
HV Gelboin,
SS Park,
GC Tsokos,
and
FK Friedman
A multiplicity of cytochromes P-450 is responsible for the detoxification and activation of xenobiotics such as drugs and carcinogens. Individual differences in sensitivity to these agents may reside in the cytochrome P-450 phenotype. A monoclonal antibody-directed radioimmunoassay was developed that detects epitope-specific cytochromes P-450 in human placentas and peripheral lymphocytes. Placentas from women who smoked cigarettes contained greater amounts of cytochrome P-450 with the monoclonal antibody-specific epitope than placentas from nonsmokers. The amount of this cytochrome P-450 in human peripheral lymphocytes increased after treatment of the mitogenized lymphocytes with the cytochrome P-450 inducer benz[a]anthracene.
