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Science 19 April 1985:
Vol. 228. no. 4697, pp. 329 - 332
DOI: 10.1126/science.2984770
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Articles

Science, Vol 228, Issue 4697, 329-332
Copyright © 1985 by American Association for the Advancement of Science

articles

Construction and recovery of viable retroviral genomes carrying a bacterial suppressor transfer RNA gene

LI Lobel, M Patel, W King, MC Nguyen-Huu, and SP Goff

The integration of retroviral genomes into cellular DNA can induce mutations by altering the expression of nearby cellular genes and can serve to identify the gene affected. The construction of a retrovirus that stably carries a suppressor transfer RNA gene from Escherichia coli has allowed facile recovery of the viral genome in vectors marked with amber mutations. This virus can be used for rapid isolation of cellular sequences at the site of proviral insertion.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Inserting a Nuclear Targeting Signal into a Replication-Competent Moloney Murine Leukemia Virus Affects Viral Export and Is Not Sufficient for Cell Cycle-Independent Infection.
J. A. Seamon, K. S. Jones, C. Miller, and M. J. Roth (2002)
J. Virol. 76, 8475-8484
   Abstract »    Full Text »    PDF »
A Replication-Competent Feline Leukemia Virus, Subgroup A (FeLV-A), Tagged with Green Fluorescent Protein Reporter Exhibits In Vitro Biological Properties Similar to Those of the Parental FeLV-A.
Z. Chang, J. Pan, C. Logg, N. Kasahara, and P. Roy-Burman (2001)
J. Virol. 75, 8837-8841
   Abstract »    Full Text »    PDF »
Genomic Stability of Murine Leukemia Viruses Containing Insertions at the Env-3' Untranslated Region Boundary.
C. R. Logg, A. Logg, C.-K. Tai, P. M. Cannon, and N. Kasahara (2001)
J. Virol. 75, 6989-6998
   Abstract »    Full Text »
Mouse Mammary Tumor Virus Carrying a Bacterial supF Gene Has Wild-Type Pathogenicity and Enables Rapid Isolation of Proviral Integration Sites.
Z. Jiang and G. M. Shackleford (1999)
J. Virol. 73, 9810-9815
   Abstract »    Full Text »
Effects of 3' Untranslated Region Mutations on Plus-Strand Priming during Moloney Murine Leukemia Virus Replication.
N. D. Robson and A. Telesnitsky (1999)
J. Virol. 73, 948-957
   Abstract »    Full Text »
A nucleoprotein complex mediates the integration of retroviral DNA..
B Bowerman, P O Brown, J M Bishop, and H E Varmus (1989)
Genes & Dev. 3, 469-478
   Abstract »    PDF »
Insertion mutagenesis of embryonal carcinoma cells by retroviruses.
W King, M. Patel, L. Lobel, S. Goff, and M. Nguyen-Huu (1985)
Science 228, 554-558
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)