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Science 19 April 1985:
Vol. 228. no. 4697, pp. 285 - 291
DOI: 10.1126/science.228.4697.285
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Articles

New Ways to Study Developmental Genes in Spore-Forming Bacteria

Philip Youngman 1, Peter Zuber 2, John B. Perkins 2, Kathleen Sandman 3, Michele Igo 3, and Richard Losick 4

1 Research associate Department of Cellular and Developmental Biology, The Biological Laboratories, Harvard University, Cambridge, Massachusetts, 02138.
2 Postdoctoral fellows Department of Cellular and Developmental Biology, The Biological Laboratories, Harvard University, Cambridge, Massachusetts, 02138.
3 Graduate students Department of Cellular and Developmental Biology, The Biological Laboratories, Harvard University, Cambridge, Massachusetts, 02138.
4 Professor and chairman in the Department of Cellular and Developmental Biology, The Biological Laboratories, Harvard University, Cambridge, Massachusetts, 02138.

The regulated activation of numerous sets of genes in multiple chromosomal locations is a hallmark of cellular differentiation in both eukaryotes and prokaryotes. Certain species of bacteria that experience complex developmental cycles are especially attractive as systems in which to study the mechanisms of this kind of gene regulation because they are highly amenable to both biochemical and genetic approaches. Bacillus subtilis, which undergoes extensive cellular differentiation when it sporulates, is one such system. Many new methods are now available in this Gram-positive species for identifying, manipulating, and studying the regulation of genes involved in spore formation, including the use of transposable genetic elements that create gene fusions in vivo as an automatic consequence of insertions into genes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
In Vitro Mutagenesis of Bacillus subtilis by Using a Modified Tn7 Transposon with an Outward-Facing Inducible Promoter.
C. Bordi, B. G. Butcher, Q. Shi, A.-B. Hachmann, J. E. Peters, and J. D. Helmann (2008)
Appl. Envir. Microbiol. 74, 3419-3425
   Abstract »    Full Text »    PDF »
Unmasking Novel Sporulation Genes in Bacillus subtilis.
J. M. Silvaggi, D. L. Popham, A. Driks, P. Eichenberger, and R. Losick (2004)
J. Bacteriol. 186, 8089-8095
   Abstract »    Full Text »    PDF »
Escherichia coli genes regulated by cell-to-cell signaling.
R. R. Baca-DeLancey, M. M. T. South, X. Ding, and P. N. Rather (1999)
PNAS 96, 4610-4614
   Abstract »    Full Text »    PDF »
Strategies and applications of in vitro mutagenesis.
D Botstein and D Shortle (1985)
Science 229, 1193-1201
   PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)