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Science 22 March 1985:
Vol. 227. no. 4693, pp. 1490 - 1492
DOI: 10.1126/science.2983428
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Articles

Science, Vol 227, Issue 4693, 1490-1492
Copyright © 1985 by American Association for the Advancement of Science

articles

Protection from genital herpes simplex virus type 2 infection by vaccination with cloned type 1 glycoprotein D

PW Berman, T Gregory, D Crase, and LA Lasky

Guinea pigs were vaccinated with truncated herpes simplex virus type-1 (HSV-1) glycoprotein D produced in the genetically engineered mammalian cell line gD10.2. Vaccinated animals formed antibodies that neutralized both HSV-1 and herpes simplex virus type 2 (HSV-2) in an in vitro neutralization assay. Vaccinated animals were challenged with HSV-2 by intravaginal infection. Animals that received the immunogen in Freund's complete adjuvant were completely protected from the clinical manifestations of genital HSV-2 infection. Animals that received the immunogen incorporated in alum adjuvants were partly protected from clinical disease; the infections that did develop were significantly less severe than those that occurred in control animals injected with adjuvant alone. The results demonstrate that immunization with a purified viral protein can provide significant protection against primary genital infection by HSV-2 in guinea pigs.


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Protective immunity against lethal HSV-1 challenge in mice by nucleic acid-based immunisation with herpes simplex virus type-1 genes specifying glycoproteins gB and gD.
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