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Science 8 March 1985:
Vol. 227. no. 4691, pp. 1174 - 1179
DOI: 10.1126/science.3975607
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Articles

Science, Vol 227, Issue 4691, 1174-1179
Copyright © 1985 by American Association for the Advancement of Science

articles

Transformation of human bronchial epithelial cells transfected by Harvey ras oncogene

GH Yoakum, JF Lechner, EW Gabrielson, BE Korba, L Malan-Shibley, JC Willey, MG Valerio, AM Shamsuddin, BF Trump, and CC Harris

Transfection of normal human bronchial epithelial (NHBE) cells with a plasmid carrying the ras oncogene of Harvey murine sarcoma virus (v-Ha ras) changed the growth requirements, terminal differentiation, and tumorigenicity of the recipient cells. One of the cell lines isolated after transfection (TBE-1) was studied extensively and shown to contain v-Ha ras DNA. Total cellular RNA from TBE-1 cells hybridized to v-Ha ras structural gene fragment probes five to eight times more than RNA from parental NHBE cells. The TBE-1 cells expressed phosphorylated v-Ha ras polypeptide p21, showed a reduced requirement for growth-factor supplements, and became aneuploid as an early cellular response to v-Ha ras expression. As the transfectants acquire an indefinite life-span and anchorage independence they became transplantable tumor cells and showed many phenotypic changes suggesting a pleiotropic mechanism for the role of Ha ras in human carcinogenesis.


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Rules for Making Human Tumor Cells.
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Intercellular Communication in Bronchial Epithelial Cells: Review of Evidence for a Possible Role in Lung Carcinogenesis.
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Primary rat embryo cells transformed by one or two oncogenes show different metastatic potentials.
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