Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 25 January 1985:
Vol. 227. no. 4685, pp. 440 - 442
DOI: 10.1126/science.3880923
Prev | Table of Contents

Articles

Science, Vol 227, Issue 4685, 440-442
Copyright © 1985 by American Association for the Advancement of Science

articles

Complete development of hepatic stages of Plasmodium falciparum in vitro

D Mazier, RL Beaudoin, S Mellouk, P Druilhe, B Texier, J Trosper, F Miltgen, I Landau, C Paul, O Brandicourt, and al. et

An in vitro model was developed to study the hepatic phase of Plasmodium falciparum, the only malaria parasite lethal to man. Primary cultures of human hepatocytes were inoculated with sporozoites of Brazilian and African strains of P. falciparum. On days 1 through 7 after inoculation examination of fluorescence-labeled and Giemsa-stained preparations demonstrated the presence of many intracellular parasites. In three separate sets of experiments all cultures were found to be infected with as many as 650 liver schizonts measuring up to 40 micrometers. After the addition of red blood cells, intraerythrocytic forms of P. falciparum were detected on days 12 and 13 by an immunofluorescence assay, indicating that the hepatic cycle had been completed in vitro.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cell therapy for the diseased liver: from stem cell biology to novel models for hepatotropic human pathogens.
N. Brezillon, D. Kremsdorf, and M. C. Weiss (2008)
Dis. Model. Mech. 1, 113-130
   Abstract »    Full Text »    PDF »
Primary Infection of C57BL/6 Mice with Plasmodium yoelii Induces a Heterogeneous Response of NKT Cells.
V. Soulard, J. Roland, C. Sellier, A. C. Gruner, M. Leite-de-Moraes, J.-F. Franetich, L. Renia, P.-A. Cazenave, and S. Pied (2007)
Infect. Immun. 75, 2511-2522
   Abstract »    Full Text »    PDF »
Malaria liver stage culture: in vitro veritas?.
S. H. I. KAPPE and P. E. DUFFY (2006)
Am J Trop Med Hyg 74, 706-707
   Full Text »    PDF »
ESTABLISHMENT OF A HUMAN HEPATOCYTE LINE THAT SUPPORTS IN VITRO DEVELOPMENT OF THE EXO-ERYTHROCYTIC STAGES OF THE MALARIA PARASITES PLASMODIUM FALCIPARUM AND P. VIVAX..
J. SATTABONGKOT, N. YIMAMNUAYCHOKE, S. LEELAUDOMLIPI, M. RASAMEESORAJ, R. JENWITHISUK, R. E. COLEMAN, R. UDOMSANGPETCH, L. CUI, and T. G. BREWER (2006)
Am J Trop Med Hyg 74, 708-715
   Abstract »    Full Text »    PDF »
New approach for high-throughput screening of drug activity on Plasmodium liver stages..
A. Gego, O. Silvie, J.-F. Franetich, K. Farhati, L. Hannoun, A. J. F. Luty, R. W. Sauerwein, C. Boucheix, E. Rubinstein, and D. Mazier (2006)
Antimicrob. Agents Chemother. 50, 1586-1589
   Abstract »    Full Text »    PDF »
A Role for Apical Membrane Antigen 1 during Invasion of Hepatocytes by Plasmodium falciparum Sporozoites.
O. Silvie, J.-F. Franetich, S. Charrin, M. S. Mueller, A. Siau, M. Bodescot, E. Rubinstein, L. Hannoun, Y. Charoenvit, C. H. Kocken, et al. (2004)
J. Biol. Chem. 279, 9490-9496
   Abstract »    Full Text »    PDF »
In Vitro Activities of 25 Quinolones and Fluoroquinolones against Liver and Blood Stage Plasmodium spp..
N. Mahmoudi, L. Ciceron, J.-F. Franetich, K. Farhati, O. Silvie, W. Eling, R. Sauerwein, M. Danis, D. Mazier, and F. Derouin (2003)
Antimicrob. Agents Chemother. 47, 2636-2639
   Abstract »    Full Text »    PDF »
Cultivation of Plasmodium spp..
F. L. Schuster (2002)
Clin. Microbiol. Rev. 15, 355-364
   Abstract »    Full Text »    PDF »
Cytokine-Sensitive Replication of Hepatitis B Virus in Immortalized Mouse Hepatocyte Cultures.
V. Pasquetto, S. F. Wieland, S. L. Uprichard, M. Tripodi, and F. V. Chisari (2002)
J. Virol. 76, 5646-5653
   Abstract »    Full Text »    PDF »
Liver CD4-CD8- NK1.1+ TCR{alpha}{beta} Intermediate Cells Increase During Experimental Malaria Infection and Are Able to Exhibit Inhibitory Activity Against the Parasite Liver Stage In Vitro.
S. Pied, J. Roland, A. Louise, D. Voegtle, V. Soulard, D. Mazier, and P.-A. Cazenave (2000)
J. Immunol. 164, 1463-1469
   Abstract »    Full Text »    PDF »
Effect of antibodies to recombinant and synthetic peptides on P. falciparum sporozoites in vitro.
D Mazier, S Mellouk, R. Beaudoin, B Texier, P Druilhe, W Hockmeyer, J Trosper, C Paul, Y Charoenvit, J Young, et al. (1986)
Science 231, 156-159
   Abstract »    PDF »
Expression of Plasmodium falciparum circumsporozoite proteins in Escherichia coli for potential use in a human malaria vaccine.
J. Young, W. Hockmeyer, M Gross, W. Ballou, R. Wirtz, J. Trosper, R. Beaudoin, M. Hollingdale, L. Miller, C. Diggs, et al. (1985)
Science 228, 958-962
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)