Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 25 January 1985:
Vol. 227. no. 4685, pp. 429 - 433
DOI: 10.1126/science.2981434
Prev | Table of Contents | Next

Articles

Science, Vol 227, Issue 4685, 429-433
Copyright © 1985 by American Association for the Advancement of Science

articles

Antibodies to peptides detect new hepatitis B antigen: serological correlation with hepatocellular carcinoma

AM Moriarty, H Alexander, RA Lerner, and GB Thornton

The expression of a previously unidentified gene product, encoded by the hepatitis B virus (HBV) genome, has been achieved with a recombinant SV40 expression vector. Antibodies against synthetic peptides representing defined regions of this protein were used to screen cells infected with recombinant virus as well as tissues naturally infected with HBV. A 24,000-dalton protein (p24) was detected in cells infected with recombinant virus and a 28,000-dalton protein (p28) was detected in tissues infected with HBV. The peptides or recombinant-derived protein were used as antigens to screen sera from individuals infected with HBV. Specific antibodies were detected predominantly in sera from patients with hepatocellular carcinoma. The presence of p28 in tissues infected with HBV and the appearance of specific antibodies in infectious sera establish the existence of an additional marker for HBV infection.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Detection of the Hepatitis B Virus X Protein (HBx) Antigen and Anti-HBx Antibodies in Cases of Human Hepatocellular Carcinoma.
G.-Y. Hwang, C.-Y. Lin, L.-M. Huang, Y.-H. Wang, J.-C. Wang, C.-T. Hsu, S.-S. Yang, and C.-C. Wu (2003)
J. Clin. Microbiol. 41, 5598-5603
   Abstract »    Full Text »    PDF »
Different Regions of Hepatitis B Virus X Protein Are Required for Enhancement of bZip-Mediated Transactivation versus Transrepression.
S. Barnabas and O. M. Andrisani (2000)
J. Virol. 74, 83-90
   Abstract »    Full Text »
Differential Immediate Early Gene Expression in Conditional Hepatitis B Virus pX-transforming Versus Nontransforming Hepatocyte Cell Lines.
C. Tarn, M. L. Bilodeau, R. L. Hullinger, and O. M. Andrisani (1999)
J. Biol. Chem. 274, 2327-2336
   Abstract »    Full Text »    PDF »
HBx Protein of Hepatitis B Virus Activates Jak1-STAT Signaling.
Y.-H. Lee and Y. Yun (1998)
J. Biol. Chem. 273, 25510-25515
   Abstract »    Full Text »    PDF »
X-gene Product of Hepatitis B Virus Induces Apoptosis in Liver Cells.
H. Kim, H. Lee, and Y. Yun (1998)
J. Biol. Chem. 273, 381-385
   Abstract »    Full Text »    PDF »
The Hepatitis B Virus X Protein Enhances the DNA Binding Potential and Transcription Efficacy of bZip Transcription Factors.
S. Barnabas, T. Hai, and O. M. Andrisani (1997)
J. Biol. Chem. 272, 20684-20690
   Abstract »    Full Text »    PDF »
Hepatocellular Carcinoma.
A. M. DI BISCEGLIE, V. K. RUSTGI, J. H. HOOFNAGLE, G. M. DUSHEIKO, and M. T. LOTZE (1988)
Ann Intern Med 108, 390-401
   Abstract »    PDF »
A transgenic mouse model of the chronic hepatitis B surface antigen carrier state.
F. Chisari, C. Pinkert, D. Milich, P Filippi, A McLachlan, R. Palmiter, and R. Brinster (1985)
Science 230, 1157-1160
   Abstract »    PDF »
Jumping Frog Genes.
B. MACDONALD (1985)
Science 227, 989
   PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)