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Science 11 January 1985:
Vol. 227. no. 4683, pp. 189 - 192
DOI: 10.1126/science.2981430
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Articles

Science, Vol 227, Issue 4683, 189-192
Copyright © 1985 by American Association for the Advancement of Science

articles

Detection of two viral genomes in single cells by double-label hybridization in situ and color microradioautography

AT Haase, D Walker, L Stowring, P Ventura, A Geballe, H Blum, M Brahic, R Goldberg, and K O'Brien

Double labeling and color microradioautography were used in a new method of hybridization in situ to identify different genes in individual cells. The method is based on the unequal penetration of 3H and 35S into two layers of nuclear track emulsion separated by a thin barrier film. Hybridization of a 35S-labeled probe specific for one kind of gene results in silver grains over cells in both layers of emulsion; a 3H-labeled probe for a second gene provides grains only in the first layer of emulsion. Silver grains are converted to magenta-colored grains in the first layer and to cyan-colored grains in the second to facilitate enumeration of grains in each layer. This technique should be widely applicable in analyses of differential gene expression in single cells or in discrete populations of cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Tissue-Specific Splicing of the Herpes Simplex Virus Type 1 Latency-Associated Transcript (LAT) Intron in LAT Transgenic Mice.
A. M. Gussow, N. V. Giordani, R. K. Tran, Y. Imai, D. L. Kwiatkowski, G. F. Rall, T. P. Margolis, and D. C. Bloom (2006)
J. Virol. 80, 9414-9423
   Abstract »    Full Text »    PDF »
Organization and Transmitter Specificity of Medullary Neurons Activated by Sustained Hypertension: Implications for Understanding Baroreceptor Reflex Circuitry.
R. K. W. Chan and P. E. Sawchenko (1998)
J. Neurosci. 18, 371-387
   Abstract »    Full Text »    PDF »
Methodologies for specific intron and exon RNA localization in cultured cells by haptenized and fluorochromized probes.
R. Dirks, F. van de Rijke, S Fujishita, M van der Ploeg, and A. Raap (1993)
J. Cell Sci. 104, 1187-1197
   Abstract »    PDF »


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