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Science 11 January 1985:
Vol. 227. no. 4683, pp. 134 - 140
DOI: 10.1126/science.3917574

Articles

Science, Vol 227, Issue 4683, 134-140
Copyright © 1985 by American Association for the Advancement of Science


articles

B lineage--specific interactions of an immunoglobulin enhancer with cellular factors in vivo

A Ephrussi, GM Church, S Tonegawa, and W Gilbert

The mouse heavy chain immunoglobulin gene contains a tissue-specific enhancer. The enhancer and flanking sequences were studied in vivo by carrying out dimethyl sulfate protection experiments on living cells, in combination with genomic sequencing. Relative to reactions on naked DNA, there are changes (protections and enhancements) in the reactivity of guanine residues to dimethyl sulfate within the enhancer sequence in myeloma, B, and early B cells, whereas virtually no alterations appear in cells of non-B lineage. Most of the affected residues are in four clusters, in sequences homologous to the octamer 5'CAGGTGGC 3' (C, cytosine; A, adenine; G. guanine; T, thymine). The alterations in the pattern of G reactivity are consistent with the tissue-specific binding of molecules to the mouse immunoglobulin heavy chain enhancer.


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Myogenin resides in the nucleus and acquires high affinity for a conserved enhancer element on heterodimerization..
T J Brennan and E N Olson (1990)
Genes & Dev. 4, 582-595
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A T cell-specific transcriptional enhancer within the human T cell receptor delta locus.
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In vivo footprinting of a muscle specific enhancer by ligation mediated PCR.
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In situ nucleoprotein structure at the SV40 major late promoter: melted and wrapped DNA flank the start site..
L Zhang and J D Gralla (1989)
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Telomeric DNA-protein interactions of Oxytricha macronuclear DNA..
C M Price and T R Cech (1987)
Genes & Dev. 1, 783-793
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In vitro binding of several cell-specific and ubiquitous nuclear proteins to the GT-I motif of the SV40 enhancer..
J H Xiao, I Davidson, M Macchi, R Rosales, M Vigneron, A Staub, and P Chambon (1987)
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Protein-binding sites in Ig gene enhancers determine transcriptional activity and inducibility.
M Lenardo, J. Pierce, and D Baltimore (1987)
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Regulation of inducible and tissue-specific gene expression.
T Maniatis, S Goodbourn, and J. Fischer (1987)
Science 236, 1237-1245
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Protein-DNA interactions in vivo upstream of a cell cycle-regulated human H4 histone gene.
U Pauli, S Chrysogelos, G Stein, J Stein, and H Nick (1987)
Science 236, 1308-1311
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Identification of a rat liver nuclear protein that binds to the enhancer core element of three animal viruses..
P F Johnson, W H Landschulz, B J Graves, and S L McKnight (1987)
Genes & Dev. 1, 133-146
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The immunoglobulin octanucleotide: independent activity and selective interaction with enhancers.
T. Parslow, S. Jones, B Bond, and K. Yamamoto (1987)
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Two different cis-active elements transfer the transcriptional effects of both EGF and phorbol esters.
H. Elsholtz, H. Mangalam, E Potter, V. Albert, S Supowit, R. Evans, and M. Rosenfeld (1986)
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