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Science 4 January 1985:
Vol. 227. no. 4682, pp. 72 - 74
DOI: 10.1126/science.2981117
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Articles

Science, Vol 227, Issue 4682, 72-74
Copyright © 1985 by American Association for the Advancement of Science

articles

Enzyme regulation in a trypanosomatid: effect of purine starvation on levels of 3'-nucleotidase activity

M Gottlieb

Crithidia fasciculata, a nonpathogenic relative of the leishmanial and trypanosomal pathogens of humans and animals, showed a 3'-ribonucleotidase activity similar to that in Leishmania donovani. The level of 3'-nucleotidase activity in Crithidia was regulated by the availability of purines in the culture medium. Specifically, organisms obtained from culture medium depleted of purines contained elevated levels of enzyme activity compared to those grown in complete medium. The 3'-nucleotidase, located at the cell surface, may serve as a first step in purine salvage for these protozoa, which are unable to synthesize the purine ring de novo.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Xanthine Phosphoribosyltransferase from Leishmania donovani. MOLECULAR CLONING, BIOCHEMICAL CHARACTERIZATION, AND GENETIC ANALYSIS.
A. Jardim, S. E. Bergeson, S. Shih, N. Carter, R. W. Lucas, G. Merlin, P. J. Myler, K. Stuart, and B. Ullman (1999)
J. Biol. Chem. 274, 34403-34410
   Abstract »    Full Text »    PDF »
Molecular Characterization of a Hyperinducible, Surface Membrane-anchored, Class I Nuclease of a Trypanosomatid Parasite.
M. Yamage, A. Debrabant, and D. M. Dwyer (2000)
J. Biol. Chem. 275, 36369-36379
   Abstract »    Full Text »    PDF »


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