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Science 7 December 1984:
Vol. 226. no. 4679, pp. 1213 - 1215
DOI: 10.1126/science.6334360
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Articles

Science, Vol 226, Issue 4679, 1213-1215
Copyright © 1984 by American Association for the Advancement of Science

articles

Homologous recombination catalyzed by mammalian cell extracts in vitro

V Darby and F Blattner

An assay was developed to detect recombination events taking place in an in vitro reaction. Extracts of cultured mouse preB lymphocytes were found to catalyze homologous recombination between substrate DNA molecules but not site-specific recombination between cloned mouse immunoglobulin D and J genes. Addition of deoxyribonucleoside triphosphates increased the frequency of homologous recombination. This recombination activity was not observed in two differentiated lymphocyte cell lines.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A novel mechanism of somatic rearrangement predicted by a human T-cell antigen receptor beta-chain complementary DNA.
A. Duby, K. Klein, C Murre, and J. Seidman (1985)
Science 228, 1204-1206
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