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Science 7 December 1984:
Vol. 226. no. 4679, pp. 1161 - 1165
DOI: 10.1126/science.6095448
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Articles

Science, Vol 226, Issue 4679, 1161-1165
Copyright © 1984 by American Association for the Advancement of Science

articles

Characterization and molecular cloning of a human parvovirus genome

SF Cotmore and P Tattersall

The genome of the small human virus serologically associated with erythrocyte aplasia and erythema infectiosum (fifth disease) is shown to be a linear, nonpermuted, single-stranded DNA molecule with self-priming hairpin termini, properties which are characteristic of the genomes of the family Parvoviridae. This human parvovirus chromosome was molecularly cloned into bacterial plasmid vectors and the cloned DNA was used to explore its relatedness to other mammalian parvovirus serotypes by DNA:DNA hybridization. It is not related to the human adeno-associated viruses but does show a distant evolutionary relationship to genomes of the helper-independent parvoviruses of rodents. This strongly suggests that it is an autonomous parvovirus, and as such is the first example of a member of this group of common animal pathogens to cause disease in man.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Molecular and Functional Analyses of a Human Parvovirus B19 Infectious Clone Demonstrates Essential Roles for NS1, VP1, and the 11-Kilodalton Protein in Virus Replication and Infectivity..
N. Zhi, I. P. Mills, J. Lu, S. Wong, C. Filippone, and K. E. Brown (2006)
J. Virol. 80, 5941-5950
   Abstract »    Full Text »    PDF »
Reactivity of Genotype-Specific Recombinant Proteins of Human Erythrovirus B19 with Plasmas from Areas Where Genotype 1 or 3 Is Endemic.
A. Parsyan, S. Kerr, S. Owusu-Ofori, G. Elliott, and J.-P. Allain (2006)
J. Clin. Microbiol. 44, 1367-1375
   Abstract »    Full Text »    PDF »
Splice Junction Map of Simian Parvovirus Transcripts.
K. Vashisht, K. S. Faaberg, A. L. Aber, K. E. Brown, and M. G. O'Sullivan (2004)
J. Virol. 78, 10911-10919
   Abstract »    Full Text »    PDF »
Advances in the biology, diagnosis and host-pathogen interactions of parvovirus B19.
A. Corcoran and S. Doyle (2004)
J. Med. Microbiol. 53, 459-475
   Abstract »    Full Text »    PDF »
Parvovirus B19.
N. S. Young and K. E. Brown (2004)
N. Engl. J. Med. 350, 586-597
   Full Text »    PDF »
Possible Interactions between the NS-1 Protein and Tumor Necrosis Factor Alpha Pathways in Erythroid Cell Apoptosis Induced by Human Parvovirus B19.
N. Sol, J. Le Junter, I. Vassias, J. M. Freyssinier, A. Thomas, A. F. Prigent, B. B. Rudkin, S. Fichelson, and F. Morinet (1999)
J. Virol. 73, 8762-8770
   Abstract »    Full Text »    PDF »
Replication of the B19 parvovirus in human bone marrow cell cultures.
K Ozawa, G Kurtzman, and N Young (1986)
Science 233, 883-886
   Abstract »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)