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Science 23 November 1984:
Vol. 226. no. 4677, pp. 972 - 974
DOI: 10.1126/science.6209798
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Articles

Science, Vol 226, Issue 4677, 972-974
Copyright © 1984 by American Association for the Advancement of Science

articles

Detection of c-sis transcripts and synthesis of PDGF-like proteins by human osteosarcoma cells

DT Graves, AJ Owen, RK Barth, P Tempst, A Winoto, L Fors, LE Hood, and HN Antoniades

Platelet-derived growth factor (PDGF) has been previously shown to be homologous to the transforming gene of simian sarcoma virus (v-sis), and inappropriate expression of the cellular counterpart of the v-sis gene (c-sis) has been implicated in the generation of mesenchymal tumors. The U-2 OS human osteosarcoma line was shown to contain multiple c-sis transcripts. Immunoprecipitation experiments with antiserum to PDGF identified a variety of polypeptides ranging in size from 18,000 to 165,000 daltons that were immunoprecipitated specifically from U-2 OS cell extracts. The osteosarcoma also was shown to secrete a 29,000-dalton protein having the serological and structural characteristics of PDGF.


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