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Science 23 November 1984:
Vol. 226. no. 4677, pp. 936 - 940
DOI: 10.1126/science.6505674
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Articles

Science, Vol 226, Issue 4677, 936-940
Copyright © 1984 by American Association for the Advancement of Science

articles

Nucleosome reconstruction via phosphorus mapping

G Harauz and FP Ottensmeyer

Electron spectroscopic imaging was combined with reconstruction algorithms to derive the three-dimensional structure of the nucleosome core particle to a resolution of 1.5 nanometers. Images of phosphorus distributions within individual nucleosomes were interpreted as projections of a supercoil of DNA. These were used to orient the corresponding individual nucleosome images, making it possible to reconstruct the entire nucleosome in three dimensions. The structure is consistent with known biochemical and biophysical data and explains site-specific nuclease sensitivity, although differing in part with other nucleosome models.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Structural States of the Nucleosome.
G. J. Czarnota and F. P. Ottensmeyer (1996)
J. Biol. Chem. 271, 3677-3683
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Science. ISSN 0036-8075 (print), 1095-9203 (online)