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Science 2 November 1984:
Vol. 226. no. 4674, pp. 559 - 561
DOI: 10.1126/science.6494901
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Articles

Science, Vol 226, Issue 4674, 559-561
Copyright © 1984 by American Association for the Advancement of Science

articles

Isolation and culture of a tetraploid subpopulation of smooth muscle cells from the normal rat aorta

ID Goldberg, EM Rosen, HM Shapiro, LC Zoller, K Myrick, SE Levenson, and L Christenson

Smooth muscle cells with 4C (double diploid) DNA content have been found in major arteries. The proportion of 4C cells increases with normal aging and with hypertension. These cells may represent a state of arrest at the G2 phase of the cell cycle or may be examples of true tetraploidy. Flow cytometric cell sorting was used to isolate 4C smooth muscle cells from the rat aorta, and the cells were cultured. Flow cytometry, Feulgen microdensitometry, and karyotyping of the progeny of the 4C cells established the presence of true tetraploid cells. These findings demonstrate the presence of reproductively viable tetraploid cells in a normal mammalian tissue.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Laminin-Binding Integrin {alpha}7 Is Required for Contractile Phenotype Expression by Human Airway Myocytes.
T. Tran, K. Ens-Blackie, E. S. Rector, G. L. Stelmack, K. D. McNeill, G. Tarone, W. T. Gerthoffer, H. Unruh, and A. J. Halayko (2007)
Am. J. Respir. Cell Mol. Biol. 37, 668-680
   Abstract »    Full Text »    PDF »
Predisposition to tetraploidy in pulmonary vascular smooth muscle cells derived from the Eker rats.
Y. Gui, G. H. He, M. P. Walsh, and X.-L. Zheng (2007)
Am J Physiol Lung Cell Mol Physiol 293, L702-L711
   Abstract »    Full Text »    PDF »


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