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Science 14 September 1984:
Vol. 225. no. 4667, pp. 1122 - 1127
DOI: 10.1126/science.6089341
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Articles

Science, Vol 225, Issue 4667, 1122-1127
Copyright © 1984 by American Association for the Advancement of Science

articles

Echinomycin binding sites on DNA

MM Van Dyke and PB Dervan

The preferred binding sites of echinomycin on DNA can be determined by a method called "footprinting." A 32P end-labeled restriction fragment from pBR322 DNA is protected by binding to echinomycin, and cleaved by a synthetic DNA cleaving reagent, methidiumpropyl--EDTA . Fe(II); the DNA cleavage products are then subjected to high-resolution gel analyses. This method reveals that echinomycin has a binding site size of four base pairs. The strong binding sites for echinomycin contain the central two-base-pair sequence 5'-CG-3'. From an analysis of 15 echinomycin sites on 210 base pairs of DNA, key recognition elements for echinomycin are contained in the sequences (5'-3') ACGT and TCGT (A, adenine; C, cytosine; G, guanine; T, thymine).


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