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Science 7 September 1984:
Vol. 225. no. 4666, pp. 993 - 998
DOI: 10.1126/science.6089340
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Articles

Science, Vol 225, Issue 4666, 993-998
Copyright © 1984 by American Association for the Advancement of Science

articles

Infectious and selectable retrovirus containing an inducible rat growth hormone minigene

AD Miller, ES Ong, MG Rosenfeld, IM Verma, and RM Evans

A growth hormone minigene carrying its natural promoter (237 nucleotides of chromosomal DNA) was stably propagated in a murine retrovirus containing hypoxanthine-guanine phosphoribosyltransferase as a selectable marker. Glucocorticoid and thyroid hormone inducibility was transferred with the growth hormone gene. Recombinant virus with titers of 10(6) per milliliter was recovered. This demonstration that retroviruses can be used to transfer a nonselectable gene under its own regulatory control enlarges the scope of retroviral vectors as potent tools for gene transfer.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Use of Intron-Disrupted Polyadenylation Sites To Enhance Expression and Safety of Retroviral Vectors.
S. I. Ismail, J. B. Rohll, S. M. Kingsman, A. J. Kingsman, and M. Uden (2001)
J. Virol. 75, 199-204
   Abstract »    Full Text »
Prospects for human gene therapy.
W. Anderson (1984)
Science 226, 401-409
   Abstract »    PDF »


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