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Science 24 August 1984:
Vol. 225. no. 4664, pp. 844 - 846
DOI: 10.1126/science.6206564
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Articles

Science, Vol 225, Issue 4664, 844-846
Copyright © 1984 by American Association for the Advancement of Science

articles

O-acetylation of disialoganglioside GD3 by human melanoma cells creates a unique antigenic determinant

DA Cheresh, RA Reisfeld, and AP Varki

Monoclonal antibody Mab D1.1 recognizes on human melanoma cells a ganglioside antigen characterized by an alkali-labile O-acetylated sialic acid residue. Immunochemical analysis showed that this molecule is an O-acetylated product of the neuroectoderm-associated disialoganglioside GD3. Controlled chemical O-acetylation of purified GD3 resulted in the generation of this same epitope. Lysates of human melanoma cells were found to contain O-acetyltransferase activity capable of generating the antigenic epitope recognized by Mab D1.1. Thus, the addition of a single O-acetyl group to a common cell surface-associated ganglioside can create a potentially tumor-specific antigen.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identification of a Sialate O-Acetyltransferase from Campylobacter jejuni: DEMONSTRATION OF DIRECT TRANSFER TO THE C-9 POSITION OF TERMINAL{alpha}-2, 8-LINKED SIALIC ACID.
R. S. Houliston, H. P. Endtz, N. Yuki, J. Li, H. C. Jarrell, M. Koga, A. van Belkum, M.-F. Karwaski, W. W. Wakarchuk, and M. Gilbert (2006)
J. Biol. Chem. 281, 11480-11486
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9-O-Acetylation of Exogenously Added Ganglioside GD3: THE GD3 MOLECULE INDUCES ITS OWN O-ACETYLATION MACHINERY.
H. Y. Chen, A. K. Challa, and A. Varki (2006)
J. Biol. Chem. 281, 7825-7833
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Genes Modulated by Expression of GD3 Synthase in Chinese Hamster Ovary Cells. EVIDENCE THAT THE Tis21 GENE IS INVOLVED IN THE INDUCTION OF GD3 9-O-ACETYLATION.
H. Satake, H. Y. Chen, and A. Varki (2003)
J. Biol. Chem. 278, 7942-7948
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Humoral Immune Response to a Therapeutic Polyvalent Cancer Vaccine After Complete Resection of Thick Primary Melanoma and Sentinel Lymphadenectomy.
M. H. Chung, R. K. Gupta, E. Hsueh, R. Essner, W. Ye, R. Yee, and D. L. Morton (2003)
J. Clin. Oncol. 21, 313-319
   Abstract »    Full Text »    PDF »
O-acetylation of GD3: An Enigmatic Modification Regulating Apoptosis?.
H. Y. Chen and A. Varki (2002)
J. Exp. Med. 196, 1529-1533
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Acetylation Suppresses the Proapoptotic Activity of GD3 Ganglioside.
F. Malisan, L. Franchi, B. Tomassini, N. Ventura, I. Condo, M. R. Rippo, A. Rufini, L. Liberati, C. Nachtigall, B. Kniep, et al. (2002)
J. Exp. Med. 196, 1535-1541
   Abstract »    Full Text »    PDF »
Suppression of Ganglioside GD3 Expression in a Rat F-11 Tumor Cell Line Reduces Tumor Growth, Angiogenesis, and Vascular Endothelial Growth Factor Production.
G. Zeng, L. Gao, S. Birklé, and R. K. Yu (2000)
Cancer Res. 60, 6670-6676
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De-N-acetyl-gangliosides in Humans: Unusual Subcellular Distribution of a Novel Tumor Antigen.
R. Chammas, J. L. Sonnenburg, N. E. Watson, T. Tai, M. G. Farquhar, N. M. Varki, and A. Varki (1999)
Cancer Res. 59, 1337-1346
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Stereochemical structures of synthesized and natural plasmalogalactosylceramides from equine brain.
Y. Yachida, M. Kashiwagi, T. Mikami, K. Tsuchihashi, T. Daino, T. Akino, and S. Gasa (1998)
J. Lipid Res. 39, 1039-1045
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Regulation of Sialic Acid 9-O-Acetylation during the Growth and Differentiation of Murine Erythroleukemia Cells.
W.-X. Shi, R. Chammas, and A. Varki (1996)
J. Biol. Chem. 271, 31517-31525
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Linkage-specific Action of Endogenous Sialic Acid O-Acetyltransferase in Chinese Hamster Ovary Cells.
W.-X. Shi, R. Chammas, and A. Varki (1996)
J. Biol. Chem. 271, 15130-15138
   Abstract »    Full Text »    PDF »
Molecular Cloning and Characterization of Lysosomal Sialic Acid O-Acetylesterase.
M. J. Guimaraes, J. F. Bazan, J. Castagnola, S. Diaz, N. G. Copeland, D. J. Gilbert, N. A. Jenkins, A. Varki, and A. Zlotnik (1996)
J. Biol. Chem. 271, 13697-13705
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Binding of Human Plasma Sialoglycoproteins by the B Cell-specific Lectin CD22.
K. Hanasaki and A. Varki (1995)
J. Biol. Chem. 270, 7543-7550
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Expression of De-N-acetyl-gangliosides in Human Melanoma Cells Is Induced by Genistein or Nocodazole.
E. R. Sjoberg, R. Chammas, H. Ozawa, I. Kawashima, K.-H. Khoo, H. R. Morris, A. Dell, T. Tai, and A. Varki (1995)
J. Biol. Chem. 270, 2921-2930
   Abstract »    Full Text »    PDF »
Selective inactivation of influenza C esterase: a probe for detecting 9-O-acetylated sialic acids.
E. Muchmore and A Varki (1987)
Science 236, 1293-1295
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