Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 17 August 1984:
Vol. 225. no. 4663, pp. 687 - 693
DOI: 10.1126/science.6431612
Prev | Table of Contents | Next

Articles

Science, Vol 225, Issue 4663, 687-693
Copyright © 1984 by American Association for the Advancement of Science

articles

Antibodies to human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation

H Persson, L Hennighausen, R Taub, W DeGrado, and P Leder

Antisera to a synthetic c-myc peptide and to c-myc antigens synthesized from various portions of the human gene expressed in Escherichia coli were used in order to characterize the protein product of the human c-myc oncogene. Although the deduced molecular weight of the human c-myc protein is 49,000, these antisera precipitate a protein from human cells that migrates in sodium dodecyl sulfate-polyacrylamide gel as if its molecular weight were 65,000. In addition, the mouse c-myc protein, whether synthesized in cells or in a cell-free system directed by pure, synthetic messenger RNA, has analogous properties and is immunoprecipitated by the antiserum to the human c-myc protein. Similar proteins are immunoprecipitated from monkey, rat, hamster, and frog cells, suggesting evolutionary conservation of antigenic structure of the c-myc protein among vertebrates. In addition, and in a manner consistent with the behavior of its messenger RNA, the immunoprecipitable c-myc protein is sharply induced by the action of mitogens on resting human T cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Epstein-Barr virus and Burkitt lymphoma.
G Brady, G J MacArthur, and P J Farrell (2008)
Postgrad. Med. J. 84, 372-377
   Abstract »    Full Text »    PDF »
Epstein Barr virus and Burkitt lymphoma.
G Brady, G J MacArthur, and P J Farrell (2007)
J. Clin. Pathol. 60, 1397-1402
   Abstract »    Full Text »    PDF »
MicroRNA Let-7a Down-regulates MYC and Reverts MYC-Induced Growth in Burkitt Lymphoma Cells.
V. B. Sampson, N. H. Rong, J. Han, Q. Yang, V. Aris, P. Soteropoulos, N. J. Petrelli, S. P. Dunn, and L. J. Krueger (2007)
Cancer Res. 67, 9762-9770
   Abstract »    Full Text »    PDF »
Mnt-Max to Myc-Max complex switching regulates cell cycle entry.
W. Walker, Z.-Q. Zhou, S. Ota, A. Wynshaw-Boris, and P. J. Hurlin (2005)
J. Cell Biol. 169, 405-413
   Abstract »    Full Text »    PDF »
Androgen-Regulated Transcripts in the Neonatal Mouse Testis as Determined Through Microarray Analysis.
Q. Zhou, J. E. Shima, R. Nie, P. J. Friel, and M. D. Griswold (2005)
Biol Reprod 72, 1010-1019
   Abstract »    Full Text »    PDF »
Altered expression of c-myc, p16 and p27 in rat colon tumors and its reversal by short-term treatment with chemopreventive agents.
L. Tao, P. M. Kramer, W. Wang, S. Yang, R. A. Lubet, V. E. Steele, and M. A. Pereira (2002)
Carcinogenesis 23, 1447-1454
   Abstract »    Full Text »    PDF »
Effect of Trichloroethylene and Its Metabolites, Dichloroacetic Acid and Trichloroacetic Acid, on the Methylation and Expression of c-Jun and c-Myc Protooncogenes in Mouse Liver: Prevention by Methionine.
L. Tao, S. Yang, M. Xie, P. M. Kramer, and M. A. Pereira (2000)
Toxicol. Sci. 54, 399-407
   Abstract »    Full Text »    PDF »
The alternatively initiated c-Myc proteins differentially regulate transcription through a noncanonical DNA-binding site..
S R Hann, M Dixit, R C Sears, and L Sealy (1994)
Genes & Dev. 8, 2441-2452
   Abstract »    PDF »
The Role of myc Oncogenes in Cell Growth and Differentiation.
T.D. Littlewood and G.I. Evan (1990)
Advances in Dental Research 4, 69-79
   Abstract »    PDF »
Constitutive expression of c-fos antisense RNA blocks c-fos gene induction by interferon and by phorbol ester and reduces c-myc expression in F9 embryonal carcinoma cells..
B Z Levi and K Ozato (1988)
Genes & Dev. 2, 554-566
   Abstract »    PDF »
Cloning of complementary DNA for GAP-43, a neuronal growth-related protein.
L. Karns, S. Ng, J. Freeman, and M. Fishman (1987)
Science 236, 597-600
   Abstract »    PDF »
Molecular Genetic Analysis of Human Lymphoid Neoplasms: Immunoglobulin Genes and the c-myc Oncogene.
T. A. WALDMANN, S. J. KORSMEYER, A. BAKHSHI, A. ARNOLD, and I. R. KIRSCH (1985)
Ann Intern Med 102, 497-510
   Abstract »    PDF »
Nuclear localization and DNA binding properties of a protein expressed by human c-myc oncogene.
H Persson and P Leder (1984)
Science 225, 718-721
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)