Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 10 August 1984:
Vol. 225. no. 4662, pp. 643 - 645
DOI: 10.1126/science.6740333
Prev | Table of Contents | Next

Articles

Science, Vol 225, Issue 4662, 643-645
Copyright © 1984 by American Association for the Advancement of Science

articles

An activated rasN gene: detected in late but not early passage human PA1 teratocarcinoma cells

MA Tainsky, CS Cooper, BC Giovanella, and GF Vande Woude

Early passages of the human teratocarcinoma cell line PA1 are not tumorigenic in nude mice, while late passages are. A transforming gene present in late passages of PA1 cells was isolated as a biologically active molecular clone and is a new isolate of the human rasN locus. Its transforming activity is due to a single G---A (G, guanine; A, adenine) point mutation at the codon for amino acid 12 which changes the codon for glycine so that an aspartic acid residue is expressed. In contrast to late passage PA1 cells (passages 106, 330, and 338), DNA from the PA1 cell line at early passages (passage 36) does not yield rasN foci in DNA transfection assays. Thus, the presence of an activated rasN in PA1 cells correlates with enhanced tumorigenicity of the cell line and, more importantly, may have arisen during cell culture in vitro.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Resveratrol Suppresses Growth of Human Ovarian Cancer Cells in Culture and in a Murine Xenograft Model: Eukaryotic Elongation Factor 1A2 as a Potential Target.
M.-H. Lee, B. Y. Choi, J. K. Kundu, Y. K. Shin, H.-K. Na, and Y.-J. Surh (2009)
Cancer Res. 69, 7449-7458
   Abstract »    Full Text »    PDF »
Coactivator PC4 Mediates AP-2 Transcriptional Activity and Suppresses ras-Induced Transformation Dependent on AP-2 Transcriptional Interference.
P. Kannan and M. A. Tainsky (1999)
Mol. Cell. Biol. 19, 899-908
   Abstract »    Full Text »    PDF »
N-ras oncogene causes AP-2 transcriptional self-interference, which leads to transformation..
P Kannan, R Buettner, P J Chiao, S O Yim, M Sarkiss, and M A Tainsky (1994)
Genes & Dev. 8, 1258-1269
   Abstract »    PDF »
Activated proto-onc genes: sufficient or necessary for cancer?.
P. Duesberg (1985)
Science 228, 669-677
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)