Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 10 August 1984:
Vol. 225. no. 4662, pp. 593 - 599
DOI: 10.1126/science.6204383
Prev | Table of Contents | Next

Articles

Science, Vol 225, Issue 4662, 593-599
Copyright © 1984 by American Association for the Advancement of Science

articles

Structure of the gene encoding the immunodominant surface antigen on the sporozoite of the human malaria parasite Plasmodium falciparum

JB Dame, JL Williams, TF McCutchan, JL Weber, RA Wirtz, WT Hockmeyer, WL Maloy, JD Haynes, I Schneider, D Roberts, and al. et

The gene for the circumsporozoite (CS) protein of Plasmodium falciparum has been cloned and its nucleotide sequence determined. The gene encodes a protein of 412 amino acids as deduced from the nucleotide sequence. The protein contains 41 tandem repeats of a tetrapeptide, 37 of which are Asn-Ala-Asn-Pro and four of which are Asn-Val-Asp-Pro. Monoclonal antibodies against the CS protein of Plasmodium falciparum were inhibited from binding to the protein by synthetic peptides of the repeat sequence. The CS protein of Plasmodium falciparum and the CS protein of a simian malaria parasite, Plasmodium knowlesi, have two regions of homology, one of which is present on either side of the repeat. One region contains 12 of 13 identical amino acids. Within the nucleotide sequence of this region, 25 of 27 nucleotides are conserved. The conservation of these regions in parasites widely separated in evolution suggests that they may have a function such as binding to liver cells and may represent an invariant target for immunity.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Evidence for Multiple B- and T-Cell Epitopes in Plasmodium falciparum Liver-Stage Antigen 3.
A. Toure-Balde, B.-L. Perlaza, J.-P. Sauzet, M. Ndiaye, G. Aribot, A. Tall, C. Sokhna, C. Rogier, G. Corradin, C. Roussilhon, et al. (2009)
Infect. Immun. 77, 1189-1196
   Abstract »    Full Text »    PDF »
Immunological Dominance of Trypanosoma cruzi Tandem Repeat Proteins.
Y. Goto, D. Carter, and S. G. Reed (2008)
Infect. Immun. 76, 3967-3974
   Abstract »    Full Text »    PDF »
Wheat Germ Cell-Free System-Based Production of Malaria Proteins for Discovery of Novel Vaccine Candidates.
T. Tsuboi, S. Takeo, H. Iriko, L. Jin, M. Tsuchimochi, S. Matsuda, E.-T. Han, H. Otsuki, O. Kaneko, J. Sattabongkot, et al. (2008)
Infect. Immun. 76, 1702-1708
   Abstract »    Full Text »    PDF »
Two Decades of Commitment to Malaria Vaccine Development: GlaxoSmithKline Biologicals.
W. R. Ballou and C. P. Cahill (2007)
Am J Trop Med Hyg 77, 289-295
   Abstract »    Full Text »    PDF »
Enhanced Immunity to Plasmodium falciparum Circumsporozoite Protein (PfCSP) by Using Salmonella enterica Serovar Typhi Expressing PfCSP and a PfCSP-Encoding DNA Vaccine in a Heterologous Prime-Boost Strategy.
M. Chinchilla, M. F. Pasetti, S. Medina-Moreno, J. Y. Wang, O. G. Gomez-Duarte, R. Stout, M. M. Levine, and J. E. Galen (2007)
Infect. Immun. 75, 3769-3779
   Abstract »    Full Text »    PDF »
RESTRICTED T-CELL EPITOPE DIVERSITY IN THE CIRCUMSPOROZOITE PROTEIN FROM PLASMODIUM FALCIPARUM POPULATIONS PREVALENT IN IRAN.
S. ZAKERI, M. AVAZALIPOOR, A. A. MEHRIZI, N. D. DJADID, and G. SNOUNOU (2007)
Am J Trop Med Hyg 76, 1046-1051
   Abstract »    Full Text »    PDF »
Bioinformatic Identification of Tandem Repeat Antigens of the Leishmania donovani Complex.
Y. Goto, R. N. Coler, and S. G. Reed (2007)
Infect. Immun. 75, 846-851
   Abstract »    Full Text »    PDF »
Developmental Biology of Sporozoite-Host Interactions in Plasmodium falciparum Malaria: Implications for Vaccine Design.
J. E. Garcia, A. Puentes, and M. E. Patarroyo (2006)
Clin. Microbiol. Rev. 19, 686-707
   Abstract »    Full Text »    PDF »
Identification of humoral immune responses in protein microarrays using DNA microarray data analysis techniques.
S. Sundaresh, D. L. Doolan, S. Hirst, Y. Mu, B. Unal, D. H. Davies, P. L. Felgner, and P. Baldi (2006)
Bioinformatics 22, 1760-1766
   Abstract »    Full Text »    PDF »
Identification and Stoichiometry of Glycosylphosphatidylinositol-anchored Membrane Proteins of the Human Malaria Parasite Plasmodium falciparum.
P. R. Gilson, T. Nebl, D. Vukcevic, R. L. Moritz, T. Sargeant, T. P. Speed, L. Schofield, and B. S. Crabb (2006)
Mol. Cell. Proteomics 5, 1286-1299
   Abstract »    Full Text »    PDF »
Sequence Variation in the T-Cell Epitopes of the Plasmodium falciparum Circumsporozoite Protein among Field Isolates Is Temporally Stable: a 5-Year Longitudinal Study in Southern Vietnam.
A. Jalloh, H. van Thien, M. U. Ferreira, J. Ohashi, H. Matsuoka, T. Kanbe, A. Kikuchi, and F. Kawamoto (2006)
J. Clin. Microbiol. 44, 1229-1235
   Abstract »    Full Text »    PDF »
An Immunologically Cryptic Epitope of Plasmodium falciparum Circumsporozoite Protein Facilitates Liver Cell Recognition and Induces Protective Antibodies That Block Liver Cell Invasion.
D. Rathore, R. Nagarkatti, D. Jani, R. Chattopadhyay, P. de la Vega, S. Kumar, and T. F. McCutchan (2005)
J. Biol. Chem. 280, 20524-20529
   Abstract »    Full Text »    PDF »
A Binding Site for Highly Sulfated Heparan Sulfate Is Identified in the N Terminus of the Circumsporozoite Protein: SIGNIFICANCE FOR MALARIAL SPOROZOITE ATTACHMENT TO HEPATOCYTES.
J. B. Ancsin and R. Kisilevsky (2004)
J. Biol. Chem. 279, 21824-21832
   Abstract »    Full Text »    PDF »
Rationale and plans for developing a non-replicating, metabolically active, radiation-attenuated Plasmodium falciparum sporozoite vaccine.
T. C. Luke and S. L. Hoffman (2003)
J. Exp. Biol. 206, 3803-3808
   Abstract »    Full Text »    PDF »
Molecular Mechanism of Host Specificity in Plasmodium falciparum Infection: ROLE OF CIRCUMSPOROZOITE PROTEIN.
D. Rathore, S. C. L. Hrstka, J. B. Sacci Jr., P. De la Vega, R. J. Linhardt, S. Kumar, and T. F. McCutchan (2003)
J. Biol. Chem. 278, 40905-40910
   Abstract »    Full Text »    PDF »
Cutting Edge: A New Tool to Evaluate Human Pre-Erythrocytic Malaria Vaccines: Rodent Parasites Bearing a Hybrid Plasmodium falciparum Circumsporozoite Protein.
C. Persson, G. A. Oliveira, A. A. Sultan, P. Bhanot, V. Nussenzweig, and E. Nardin (2002)
J. Immunol. 169, 6681-6685
   Abstract »    Full Text »    PDF »
Cryptosporidium parvum Genes Containing Thrombospondin Type 1 Domains.
M. Deng, T. J. Templeton, N. R. London, C. Bauer, A. A. Schroeder, and M. S. Abrahamsen (2002)
Infect. Immun. 70, 6987-6995
   Abstract »    Full Text »    PDF »
Current Status of Malaria and Potential for Control.
R. S. Phillips (2001)
Clin. Microbiol. Rev. 14, 208-226
   Abstract »    Full Text »    PDF »
The Cytotoxic T-Lymphocyte Epitope of the Plasmodium falciparum Circumsporozoite Protein Also Modulates the Efficiency of Receptor-Ligand Interaction with Hepatocytes.
D. Rathore and T. F. McCutchan (2000)
Infect. Immun. 68, 740-743
   Abstract »    Full Text »    PDF »
Induction of Protective Immune Responses by Immunization with Linear Multiepitope Peptides Based on Conserved Sequences from Plasmodium falciparum Antigens.
A. Bharadwaj, P. Sharma, S. K. Joshi, B. Singh, and V. S. Chauhan (1998)
Infect. Immun. 66, 3232-3241
   Abstract »    Full Text »    PDF »
Genetic Polymorphism and Natural Selection in the Malaria Parasite Plasmodium falciparum.
A. A. Escalante, A. A. Lal, and F. J. Ayala (1998)
Genetics 149, 189-202
   Abstract »    Full Text »    PDF »
Plasmodium falciparum antigenic diversity: Evidence of clonal population structure.
S. M. Rich, R. R. Hudson, and F. J. Ayala (1997)
PNAS 94, 13040-13045
   Abstract »    Full Text »    PDF »
Characterization of a Novel, Stage-specific, Invariant Surface Protein in Trypanosoma brucei Containing an Internal, Serine-rich, Repetitive Motif.
D. P. Nolan, D. G. Jackson, H. J. Windle, A. Pays, M. Geuskens, A. Michel, H. P. Voorheis, and E. Pays (1997)
J. Biol. Chem. 272, 29212-29221
   Abstract »    Full Text »    PDF »
Cell Adhesion to a Motif Shared by the Malaria Circumsporozoite Protein and Thrombospondin Is Mediated by Its Glycosaminoglycan-binding Region and Not by CSVTCG.
S. M. Gantt, P. Clavijo, X. Bai, J. D. Esko, and P. Sinnis (1997)
J. Biol. Chem. 272, 19205-19213
   Abstract »    Full Text »    PDF »
Identification and Characterization of the Protective Hepatocyte Erythrocyte Protein 17kDa Gene of Plasmodium yoelii, homolog of Plasmodium falciparum Exported Protein 1.
D. L. Doolan, R. C. Hedstrom, W. O. Rogers, Y. Charoenvit, M. Rogers, P. d. l. Vega, and S. L. Hoffman (1996)
J. Biol. Chem. 271, 17861-17868
   Abstract »    Full Text »    PDF »
Developmental Expression of a Tandemly Repeated, Proline- and Glutamine-rich Amino Acid Motif on Hyphal Surfaces of Candida albicans.
J. F. Staab, C. A. Ferrer, and P. Sundstrom (1996)
J. Biol. Chem. 271, 6298-6305
   Abstract »    Full Text »    PDF »
Anchoring of an Immunogenic Plasmodium falciparum Circumsporozoite Protein on the Surface of Dictyostelium discoideum.
C. D. Reymond, C. Beghdadi-Rais, M. Roggero, E. A. Duarte, C. Desponds, M. Bernard, D. Groux, H. Matile, C. Bron, G. Corradin, et al. (1995)
J. Biol. Chem. 270, 12941-12947
   Abstract »    Full Text »    PDF »
Cell-adhesive motif in region II of malarial circumsporozoite protein.
K. Rich, F. George 4th, J. Law, and W. Martin (1990)
Science 249, 1574-1577
   Abstract »    PDF »
Use of prior vaccinations for the development of new vaccines.
H. Etlinger, D Gillessen, H. Lahm, H Matile, H. Schonfeld, and A Trzeciak (1990)
Science 249, 423-425
   Abstract »    PDF »
Conserved repetitive epitope recognized by CD4+ clones from a malaria-immunized volunteer.
E. Nardin, D. Herrington, J Davis, M Levine, D Stuber, B Takacs, P Caspers, P Barr, R Altszuler, P Clavijo, et al. (1989)
Science 246, 1603-1606
   Abstract »    PDF »
Parasitic protozoa and helminths: biological and immunological challenges.
A. Mahmoud (1989)
Science 246, 1015-1022
   Abstract »    PDF »
Circumsporozoite protein heterogeneity in the human malaria parasite Plasmodium vivax.
R Rosenberg, R. Wirtz, D. Lanar, J Sattabongkot, T Hall, A. Waters, and C Prasittisuk (1989)
Science 245, 973-976
   Abstract »    PDF »
Amplification of a gene related to mammalian mdr genes in drug-resistant Plasmodium falciparum.
C. Wilson, A. Serrano, A Wasley, M. Bogenschutz, A. Shankar, and D. Wirth (1989)
Science 244, 1184-1186
   Abstract »    PDF »
Limited immunological recognition of critical malaria vaccine candidate antigens.
M. Good, L. Miller, S Kumar, I. Quakyi, D Keister, J. Adams, B Moss, J. Berzofsky, and R Carter (1988)
Science 242, 574-577
   Abstract »    PDF »
Oral Salmonella typhimurium vaccine expressing circumsporozoite protein protects against malaria.
J. Sadoff, W. Ballou, L. Baron, W. Majarian, R. Brey, W. Hockmeyer, J. Young, S. Cryz, J Ou, G. Lowell, et al. (1988)
Science 240, 336-338
   Abstract »    PDF »
Naturally acquired antibodies to sporozoites do not prevent malaria: vaccine development implications.
S. Hoffman, C. Oster, C. Plowe, G. Woollett, J. Beier, J. Chulay, R. Wirtz, M. Hollingdale, and M Mugambi (1987)
Science 237, 639-642
   Abstract »    PDF »
Genetic analysis of the human malaria parasite Plasmodium falciparum.
D Walliker, I. Quakyi, T. Wellems, T. McCutchan, A Szarfman, W. London, L. Corcoran, T. Burkot, and R Carter (1987)
Science 236, 1661-1666
   Abstract »    PDF »
Efficacy of murine malaria sporozoite vaccines: implications for human vaccine development.
J. Egan, J. Weber, W. Ballou, M. Hollingdale, W. Majarian, D. Gordon, W. Maloy, S. Hoffman, R. Wirtz, I Schneider, et al. (1987)
Science 236, 453-456
   Abstract »    PDF »
Construction of synthetic immunogen: use of new T-helper epitope on malaria circumsporozoite protein.
M. Good, W. Maloy, M. Lunde, H Margalit, J. Cornette, G. Smith, B Moss, L. Miller, and J. Berzofsky (1987)
Science 235, 1059-1062
   Abstract »    PDF »
Research toward malaria vaccines.
L. Miller, R. Howard, R Carter, M. Good, V Nussenzweig, and R. Nussenzweig (1986)
Science 234, 1349-1356
   Abstract »    PDF »
Identification of paramyosin as schistosome antigen recognized by intradermally vaccinated mice.
D. Lanar, E. Pearce, S. James, and A Sher (1986)
Science 234, 593-596
   Abstract »    PDF »
Sequence of the immunodominant epitope for the surface protein on sporozoites of Plasmodium vivax.
T. McCutchan, A. Lal, V. de la Cruz, L. Miller, W. Maloy, Y Charoenvit, R. Beaudoin, P Guerry, R Wistar Jr, S. Hoffman, et al. (1985)
Science 230, 1381-1383
   Abstract »    PDF »
Circumsporozoite protein of Plasmodium vivax: gene cloning and characterization of the immunodominant epitope.
D. Arnot, J. Barnwell, J. Tam, V Nussenzweig, R. Nussenzweig, and V Enea (1985)
Science 230, 815-818
   Abstract »    PDF »
Diversity of circumsporozoite antigen genes from two strains of the malarial parasite Plasmodium knowlesi.
S Sharma, P Svec, G. Mitchell, and G. Godson (1985)
Science 229, 779-782
   Abstract »    PDF »
Rationale for development of a synthetic vaccine against Plasmodium falciparum malaria.
F Zavala, J. Tam, M. Hollingdale, A. Cochrane, I Quakyi, R. Nussenzweig, and V Nussenzweig (1985)
Science 228, 1436-1440
   Abstract »    PDF »
Expression of Plasmodium falciparum circumsporozoite proteins in Escherichia coli for potential use in a human malaria vaccine.
J. Young, W. Hockmeyer, M Gross, W. Ballou, R. Wirtz, J. Trosper, R. Beaudoin, M. Hollingdale, L. Miller, C. Diggs, et al. (1985)
Science 228, 958-962
   Abstract »    PDF »
Immunogenicity of synthetic peptides from circumsporozoite protein of Plasmodium falciparum.
W. Ballou, J Rothbard, R. Wirtz, D. Gordon, J. Williams, R. Gore, I Schneider, M. Hollingdale, R. Beaudoin, W. Maloy, et al. (1985)
Science 228, 996-999
   Abstract »    PDF »
Expression of the major surface antigen of Plasmodium knowlesi sporozoites in yeast.
S Sharma and G. Godson (1985)
Science 228, 879-882
   Abstract »    PDF »
Isolation of the gene for a glycophorin-binding protein implicated in erythrocyte invasion by a malaria parasite.
J. Ravetch, J Kochan, and M Perkins (1985)
Science 227, 1593-1597
   Abstract »    PDF »
Human malarial gene cloned.
J. Marx (1984)
Science 225, 607-608
   PDF »
Mung bean nuclease cleaves Plasmodium genomic DNA at sites before and after genes.
T. McCutchan, J. Hansen, J. Dame, and J. Mullins (1984)
Science 225, 625-628
   Abstract »    PDF »
Stage-dependent Localization of a Novel Gene Product of the Malaria Parasite, Plasmodium falciparum.
T. V. Nguyen, H. Fujioka, A. S. Kang, W. O. Rogers, D. A. Fidock, and A. A. James (2001)
J. Biol. Chem. 276, 26724-26731
   Abstract »    Full Text »    PDF »
Evidence for clonal propagation in natural isolates of Plasmodium falciparum from Venezuela.
L. Urdaneta, A. Lal, C. Barnabe, B. Oury, I. Goldman, F. J. Ayala, and M. Tibayrenc (2001)
PNAS 98, 6725-6729
   Abstract »    Full Text »    PDF »
Role of cysteines in Plasmodium falciparum circumsporozoite protein: Interactions with heparin can rejuvenate inactive protein mutants.
D. Rathore and T. F. McCutchan (2000)
PNAS 97, 8530-8535
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)