Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 27 July 1984:
Vol. 225. no. 4660, pp. 424 - 427
DOI: 10.1126/science.6204381
Prev | Table of Contents | Next

Articles

Science, Vol 225, Issue 4660, 424-427
Copyright © 1984 by American Association for the Advancement of Science

articles

Sindbis virus mutants selected for rapid growth in cell culture display attenuated virulence in animals

RA Olmsted, RS Baric, BA Sawyer, and RE Johnston

Mutants of Sindbis virus were selected for rapid growth in baby hamster kidney (BHK) cell cultures and screened for attenuation of virulence in suckling mice. Comparisons among independently isolated virulent and attenuated strains, as well as a classical reversion analysis, showed that accelerated penetration of BHK cells was correlated with attenuation in vivo. Both phenotypic changes resulted from a reorganization of virion structure as detected by monoclonal antibodies. These results suggest that mutants selected for rapid growth in cell culture may be useful as attenuated vaccines and for studies of the molecular basis of virus pathogenesis.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Differential Induction of Type I Interferon Responses in Myeloid Dendritic Cells by Mosquito and Mammalian-Cell-Derived Alphaviruses.
R. S. Shabman, T. E. Morrison, C. Moore, L. White, M. S. Suthar, L. Hueston, N. Rulli, B. Lidbury, J. P.-Y. Ting, S. Mahalingam, et al. (2007)
J. Virol. 81, 237-247
   Abstract »    Full Text »    PDF »
Infectivity variation and genetic diversity among strains of Western equine encephalitis virus..
L. P. Nagata, W.-G. Hu, M. Parker, D. Chau, G. A. Rayner, F. L. Schmaltz, and J. P. Wong (2006)
J. Gen. Virol. 87, 2353-2361
   Abstract »    Full Text »    PDF »
A Single Mutation in the E2 Glycoprotein Important for Neurovirulence Influences Binding of Sindbis Virus to Neuroblastoma Cells.
P. Lee, R. Knight, J. M. Smit, J. Wilschut, and D. E. Griffin (2002)
J. Virol. 76, 6302-6310
   Abstract »    Full Text »    PDF »
Differential Evolution of Eastern Equine Encephalitis Virus Populations in Response to Host Cell Type.
L. A. Cooper and T. W. Scott (2001)
Genetics 157, 1403-1412
   Abstract »    Full Text »
Cost of Host Radiation in an RNA Virus.
P. E. Turner and S. F. Elena (2000)
Genetics 156, 1465-1470
   Abstract »    Full Text »
Experimental Evolution of Parasites.
D. Ebert (1998)
Science 282, 1432-1436
   Abstract »    Full Text »
Adaptation of Sindbis Virus to BHK Cells Selects for Use of Heparan Sulfate as an Attachment Receptor.
W. B. Klimstra, K. D. Ryman, and R. E. Johnston (1998)
J. Virol. 72, 7357-7366
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)