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Science 6 July 1984:
Vol. 225. no. 4657, pp. 63 - 66
DOI: 10.1126/science.6328661
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Articles

Science, Vol 225, Issue 4657, 63-66
Copyright © 1984 by American Association for the Advancement of Science

articles

Adaptation of lymphadenopathy associated virus (LAV) to replication in EBV-transformed B lymphoblastoid cell lines

L Montagnier, J Gruest, S Chamaret, C Dauguet, C Axler, D Guetard, MT Nugeyre, F Barre-Sinoussi, JC Chermann, JB Brunet, and al. et

A strain of lymphadenopathy associated retrovirus ( LAV ) passaged in vitro was used to infect a lymphoblastoid cell line obtained by transformation with Epstein-Barr virus of B lymphocytes from a healthy donor. The virus produced from this line (B- LAV ) was also able to grow at a high rate in some other lymphoblastoid lines and in a Burkitt lymphoma line. This adapted strain retained the biochemical, ultrastructural, and antigenic characteristics of the original strain, as well as its tropism for normal T4+ lymphocytes. It is thus possible to grow LAV in large quantities that can be used for the preparation of diagnostic reagents. The interaction between such a human retrovirus and Epstein-Barr virus, a DNA virus, may have some implication for the pathology of the acquired immunodeficiency syndrome and related diseases.


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