Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Active Motif, Inc.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 22 June 1984:
Vol. 224. no. 4655, pp. 1343 - 1346
DOI: 10.1126/science.6374895
Prev | Table of Contents | Next

Articles

Science, Vol 224, Issue 4655, 1343-1346
Copyright © 1984 by American Association for the Advancement of Science

articles

Adenovirus E1a gene product expressed at high levels in Escherichia coli is functional

B Ferguson, N Jones, J Richter, and M Rosenberg

The human type C adenovirus E1a 13S messenger RNA encodes a gene product, that positively regulates the transcription of viral genes and certain cellular genes and is involved in the transformation of primary mammalian cells. The E1a gene product was expressed at high levels in Escherichia coli. In a Xenopus oocyte microinjection assay, the purified Escherichia coli-produced protein activated the E1a-responsive adenovirus E3 promoter and functioned as efficiently as the E1a gene itself.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage..
C R Vinson, K L LaMarco, P F Johnson, W H Landschulz, and S L McKnight (1988)
Genes & Dev. 2, 801-806
   Abstract »    PDF »
Identification of factors that interact with the E1A-inducible adenovirus E3 promoter..
H C Hurst and N C Jones (1987)
Genes & Dev. 1, 1132-1146
   Abstract »    PDF »
Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis.
S Rogers, R Wells, and M Rechsteiner (1986)
Science 234, 364-368
   Abstract »    PDF »
Identification of the protein encoded by the E6 transforming gene of bovine papillomavirus.
E. Androphy, J. Schiller, and D. Lowy (1985)
Science 230, 442-445
   Abstract »    PDF »
Expression of Plasmodium falciparum circumsporozoite proteins in Escherichia coli for potential use in a human malaria vaccine.
J. Young, W. Hockmeyer, M Gross, W. Ballou, R. Wirtz, J. Trosper, R. Beaudoin, M. Hollingdale, L. Miller, C. Diggs, et al. (1985)
Science 228, 958-962
   Abstract »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)