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Science 22 June 1984:
Vol. 224. no. 4655, pp. 1343 - 1346
DOI: 10.1126/science.6374895
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Articles

Science, Vol 224, Issue 4655, 1343-1346
Copyright © 1984 by American Association for the Advancement of Science

articles

Adenovirus E1a gene product expressed at high levels in Escherichia coli is functional

B Ferguson, N Jones, J Richter, and M Rosenberg

The human type C adenovirus E1a 13S messenger RNA encodes a gene product, that positively regulates the transcription of viral genes and certain cellular genes and is involved in the transformation of primary mammalian cells. The E1a gene product was expressed at high levels in Escherichia coli. In a Xenopus oocyte microinjection assay, the purified Escherichia coli-produced protein activated the E1a-responsive adenovirus E3 promoter and functioned as efficiently as the E1a gene itself.


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