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Science 15 June 1984:
Vol. 224. no. 4654, pp. 1249 - 1251
DOI: 10.1126/science.6729453
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Articles

Science, Vol 224, Issue 4654, 1249-1251
Copyright © 1984 by American Association for the Advancement of Science

articles

Enzymatic catalysis in organic media at 100 degrees C

A Zaks and AM Klibanov

Porcine pancreatic lipase catalyzes the transesterification reaction between tributyrin and various primary and secondary alcohols in a 99 percent organic medium. Upon further dehydration, the enzyme becomes extremely thermostable. Not only can the dry lipase withstand heating at 100 degrees C for many hours, but it exhibits a high catalytic activity at that temperature. Reduction in water content also alters the substrate specificity of the lipase: in contrast to its wet counterpart, the dry enzyme does not react with bulky tertiary alcohols.


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