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Science 8 June 1984:
Vol. 224. no. 4653, pp. 1095 - 1097
DOI: 10.1126/science.6585956
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Articles

Science, Vol 224, Issue 4653, 1095-1097
Copyright © 1984 by American Association for the Advancement of Science

articles

A nitrogen pressure of 50 atmospheres does not prevent evolution of hydrogen by nitrogenase

FB Simpson and RH Burris

The effect of a partial pressure of nitrogen of 50 atmospheres (5065 kilopascals ) on the hydrogen evolution reaction of nitrogenase has been investigated. Evolution of hydrogen was not blocked completely by 50 atmospheres of nitrogen in any of four experiments; rather, 27.3 +/- 2.4 percent of the total electron flux through nitrogenase was directed toward production of hydrogen. The ratio of hydrogen evolved to nitrogen fixed was close to 1:1, which implies that hydrogen evolution is obligatory in the fixation of molecular nitrogen by nitrogenase.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)