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Science 1 June 1984:
Vol. 224. no. 4652, pp. 998 - 1001
DOI: 10.1126/science.6719130
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Articles

Science, Vol 224, Issue 4652, 998-1001
Copyright © 1984 by American Association for the Advancement of Science

articles

Dynamic heterogeneity: rapid generation of metastatic variants in mouse B16 melanoma cells

RP Hill, AF Chambers, V Ling, and JF Harris

The ability of clonally derived lines of B16F1 and B16F10 melanoma cells to form experimental metastases in C57BL mice after intravenous injection was examined. Luria- Delbruck fluctuation analysis was applied to the results obtained with parallel subclones grown to small population sizes before testing for metastatic ability. The analysis demonstrated that variant cells capable of forming experimental metastases were generated in B16F1 cell populations at an effective rate of about 1.3 X 10(-5) per cell per generation while in B16F10 cell populations the effective rate of production was about 5 X 10(-5) per cell per generation. These results are consistent with a dynamic heterogeneity model of tumor progression. They suggest that the majority of cells in both lines are effectively nonmetastatic and that the higher metastatic ability of the B16F10 population may be due in part to a higher rate of generation of metastatic variants.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Identifying Cancer Stem Cells in Solid Tumors: Case Not Proven.
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Cancer Res. 66, 1891-1896
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The Guanine Nucleotide Exchange Factor CNrasGEF Regulates Melanogenesis and Cell Survival in Melanoma Cells.
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J. Biol. Chem. 281, 121-128
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Inhibition of the Motility and Growth of B16F10 Mouse Melanoma Cells by Dominant Negative Mutants of Dok-1.
T. Hosooka, T. Noguchi, H. Nagai, T. Horikawa, T. Matozaki, M. Ichihashi, and M. Kasuga (2001)
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A Pathogenic Role of Th2 Cells and Their Cytokine Products on the Pulmonary Metastasis of Murine B16 Melanoma.
M. Kobayashi, H. Kobayashi, R. B. Pollard, and F. Suzuki (1998)
J. Immunol. 160, 5869-5873
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