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Science 25 May 1984:
Vol. 224. no. 4651, pp. 876 - 879
DOI: 10.1126/science.6719116
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Articles

Science, Vol 224, Issue 4651, 876-879
Copyright © 1984 by American Association for the Advancement of Science

articles

Estrogen receptor analysis by flow cytometry

NT Van, M Raber, GH Barrows, and B Barlogie

A fluorescently labeled estradiol, N'-fluoresceino-N'-(17 beta-estradiol hemisuccinamide) thiourea (FE) was used for measuring estrogen receptor content per cell in tumor cells. The cellular content of FE was measured quantitatively by flow cytometry. Binding of FE occurs in the nanomolar concentration range, an indication of the high affinity of the labeled estradiol. Competition of FE for binding sites is observed with estrogens, but not with progestins, androgens, or glucocorticosteroids, indicating the specificity of FE binding. In contrast to other estrogen receptor assays, this new technique requires a small sample size (about 5000 cells) and permits the assessment of heterogeneity in estrogen receptor expression among tumor cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Direct Measure of Fluorescence Intensity for Efficient Receptor-binding Assay: Conjugates of Ethinylcarboxyestradiol and 5(and 6)-Carboxyfluorescein via {alpha},{omega}-Diaminoalkanes as a Tracer for Estrogen Receptor.
D. Asai, T. Tokunaga, K. Kondo, T. Kawaguchi, S. Takayanagi, T. Shinmyozu, M. Nakai, Y. Yakabe, and Y. Shimohigashi (2008)
J. Biochem. 143, 781-792
   Abstract »    Full Text »    PDF »
Oestrogen and Progesterone Hormone Receptors in Dupuytren's Disease.
F. M. HANKIN, J. ECKENRODE, and D. S. LOUIS (1986)
J Hand Surg Eur Vol. 11, 463-464
   Abstract »    PDF »


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