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Science 25 May 1984:
Vol. 224. no. 4651, pp. 843 - 848
DOI: 10.1126/science.6326261
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Articles

Science, Vol 224, Issue 4651, 843-848
Copyright © 1984 by American Association for the Advancement of Science

articles

Expression cloning of human EGF receptor complementary DNA: gene amplification and three related messenger RNA products in A431 cells

CR Lin, WS Chen, W Kruiger, LS Stolarsky, W Weber, RM Evans, IM Verma, GN Gill, and MG Rosenfeld

In order to further define the mechanisms by which polypeptide growth factors regulate gene transcription and cellular growth, expression cloning techniques were used to select human epidermal growth factor (EGF) receptor complementary DNA clones. The EGF 3' coding domain shows striking homology to the transforming gene product of avian erythroblastosis virus (v-erbB). Over-expression of EGF receptors in A431 cell lines correlates with increased EGF receptor mRNA levels and amplification (up to 110 times) of the apparently singular EGF receptor gene. There appear to be three cytoplasmic polyadenylated RNA products of EGF receptor gene expression in A431 cells, one of which contains only 5' (EGF binding domain) sequences and is postulated to encode the secreted EGF receptor-related protein.


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