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Science 27 April 1984:
Vol. 224. no. 4647, pp. 409 - 411
DOI: 10.1126/science.224.4647.409
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Articles

Analysis of Hydrothermal Vent-Associated Symbionts by Ribosomal RNA Sequences

DAVID A. STAHL 1, DAVID J. LANE 1, GARY J. OLSEN 1, and NORMAN R. PACE 1

1 National Jewish Hospital and Research Center and University of Colorado Health Sciences Center, 3800 East Colfax Avenue, Denver 80206

Ribosomal RNA (rRNA) sequences were used to establish the phylogenetic affiliations of symbioses in which prokaryotes appear to confer sulfur-based chemoautotrophy on their invertebrate hosts. Two submarine hydrothermal vent animals, the vestimentiferan tube worm Riftia pachyptila and the clam Calyptogena magnifica, and a tidal-flat bivalve, Solemya velum, were inspected. 5S rRNA's were extracted from symbiont-bearing tissues, separated into unique forms, and their nucleotide sequences determined and related to other 5S rRNA's in a phylogenetic tree analysis. The prokaryotic symbionts are related to one another and affiliated with the same narrow phylogenetic grouping as Escherichia coli and Pseudomonas aeruginosa. The sequence comparisons suggest that Riftia is more closely related to the bivalves than their current taxonomic status would suggest.

Submitted on December 21, 1983
Accepted on March 1, 1984


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