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Science 27 April 1984:
Vol. 224. no. 4647, pp. 397 - 399
DOI: 10.1126/science.6200932
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Articles

Science, Vol 224, Issue 4647, 397-399
Copyright © 1984 by American Association for the Advancement of Science

articles

Plasmodium knowlesi sporozoite antigen: expression by infectious recombinant vaccinia virus

GL Smith, GN Godson, V Nussenzweig, RS Nussenzweig, J Barnwell, and B Moss

The gene coding for the circumsporozoite antigen of the malaria parasite Plasmodium knowlesi was inserted into the vaccinia virus genome under the control of a defined vaccinia virus promoter. Cells infected with the recombinant virus synthesized polypeptides of 53,000 to 56,000 daltons that reacted with monoclonal antibody against the repeating epitope of the malaria protein. Furthermore, rabbits vaccinated with the recombinant virus produced antibodies that bound specifically to sporozoites. These data provide evidence for expression of a cloned malaria gene in mammalian cells and illustrate the potential of vaccinia virus recombinants as live malaria vaccines.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Recombinant vaccinia virus: immunization against multiple pathogens.
M. Perkus, A Piccini, B. Lipinskas, and E Paoletti (1985)
Science 229, 981-984
   Abstract »    PDF »
Expression of the major surface antigen of Plasmodium knowlesi sporozoites in yeast.
S Sharma and G. Godson (1985)
Science 228, 879-882
   Abstract »    PDF »
Vaccinia virus recombinants: expression of VSV genes and protective immunization of mice and cattle.
M Mackett, T Yilma, J. Rose, and B Moss (1985)
Science 227, 433-435
   Abstract »    PDF »


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