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Science 27 April 1984:
Vol. 224. no. 4647, pp. 392 - 395
DOI: 10.1126/science.6200931
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Articles

Science, Vol 224, Issue 4647, 392-395
Copyright © 1984 by American Association for the Advancement of Science

articles

Location and chemical synthesis of a pre-S gene coded immunodominant epitope of hepatitis B virus

AR Neurath, SB Kent, and N Strick

Immunodominant, disulfide-bond independent epitopes recognized by human antibodies to hepatitis B virus (HBV) are located within the 55-residue amino terminal portion (coded for by the pre-S region of HBV DNA) of minor HBV envelope components larger than the major protein constituents encoded by the S gene. A peptide having the sequence of the first 26 amino acids from the amino terminal methionine was synthesized and elicited antibodies (at dilutions of greater than or equal to 1 to 10(5) ) to the HBV envelope. These antibodies can be utilized for diagnostic tests. The immunogenicity of the peptide was substantially increased by covalent attachment to liposomes. The disulfide bond-independent determinants on sequences coded for by the pre-S gene may be more easily mimicked by peptide analogs than "conformational" determinants on the S-gene product.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Expression of the Human Hepatitis B Virus Large Surface Antigen Gene in Transgenic Tomato Plants.
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Mapping of immunodominant B-cell epitopes and the human serum albumin-binding site in natural hepatitis B virus surface antigen of defined genosubtype.
D. Sobotta, I. Sominskaya, J. Jansons, H. Meisel, S. Schmitt, K.-H. Heermann, G. Kaluza, P. Pumpens, and W. H. Gerlich (2000)
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Immune response to a new hepatitis B vaccine in healthcare workers who had not responded to standard vaccine: randomised double blind dose-response study.
J. N Zuckerman, C. Sabin, F. M Craig, A Williams, and A. J Zuckerman (1997)
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The nucleocapsid of hepatitis B virus is both a T-cell-independent and a T-cell-dependent antigen.
D. Milich and A McLachlan (1986)
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A transgenic mouse model of the chronic hepatitis B surface antigen carrier state.
F. Chisari, C. Pinkert, D. Milich, P Filippi, A McLachlan, R. Palmiter, and R. Brinster (1985)
Science 230, 1157-1160
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Antibodies to Hepatitis B Surface Antigen as the Sole Hepatitis B Marker in Hospital Personnel.
H. A. KESSLER, A. A. HARRIS, J. A. PAYNE, E. HUDSON, B. POTKIN, and S. LEVIN (1985)
Ann Intern Med 103, 21-26
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Enhanced immunogenicity of the pre-S region of hepatitis B surface antigen.
D. Milich, G. Thornton, A. Neurath, S. Kent, M. Michel, P Tiollais, and F. Chisari (1985)
Science 228, 1195-1199
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