Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 20 April 1984:
Vol. 224. no. 4646, pp. 294 - 297
DOI: 10.1126/science.6324343
Prev | Table of Contents | Next

Articles

Science, Vol 224, Issue 4646, 294-297
Copyright © 1984 by American Association for the Advancement of Science

articles

Production of an epidermal growth factor receptor-related protein

W Weber, GN Gill, and J Spiess

Human epidermoid carcinoma A431 cells in culture produce a soluble 105-kilodalton protein which, by the criteria of epidermal growth factor (EGF) binding, recognition by monoclonal and polyclonal antibodies to the EGF receptor, amino-terminal sequence analysis and carbohydrate content, is related to the cell surface domain of the EGF receptor. The high rate of production and the finding that with biosynthetic labeling the specific activity of this 105-kilodalton protein exceeds that of the intact receptor indicate that it is not derived from membrane-bound mature receptor but is separately produced by the cell. These cells thus separately synthesize an EGF receptor that is inserted into the membrane and an EGF receptor-related protein that is secreted.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Serum Soluble Epidermal Growth Factor Receptor Concentrations Decrease in Postmenopausal Metastatic Breast Cancer Patients Treated with Letrozole.
J. M. Lafky, A. T. Baron, E. M. Cora, D. W. Hillman, V. J. Suman, E. A. Perez, J. N. Ingle, and N. J. Maihle (2005)
Cancer Res. 65, 3059-3062
   Abstract »    Full Text »    PDF »
Decorin Binds to a Narrow Region of the Epidermal Growth Factor (EGF) Receptor, Partially Overlapping but Distinct from the EGF-binding Epitope.
M. Santra, C. C. Reed, and R. V. Iozzo (2002)
J. Biol. Chem. 277, 35671-35681
   Abstract »    Full Text »    PDF »
A Preliminary Study of Serum Concentrations of Soluble Epidermal Growth Factor Receptor (sErbB1), Gonadotropins, and Steroid Hormones in Healthy Men and Women.
A. T. Baron, J. M. Lafky, V. J. Suman, D. W. Hillman, M. C. Buenafe, C. H. Boardman, K. C. Podratz, E. A. Perez, and N. J. Maihle (2001)
Cancer Epidemiol. Biomarkers Prev. 10, 1175-1185
   Abstract »    Full Text »    PDF »
Characterization of the carbohydrate chains of the secreted form of the human epidermal growth factor receptor.
C. J.M. Stroop, W. Weber, G. J. Gerwig, M. Nimtz, J. P. Kamerling, and J. F.G. Vliegenthart (2000)
Glycobiology 10, 901-917
   Abstract »    Full Text »    PDF »
Epidermal growth factor system regulates mucin production in airways.
K. Takeyama, K. Dabbagh, H.-M. Lee, C. Agusti, J. A. Lausier, I. F. Ueki, K. M. Grattan, and J. A. Nadel (1999)
PNAS 96, 3081-3086
   Abstract »    Full Text »    PDF »
Decorin Is a Biological Ligand for the Epidermal Growth Factor Receptor.
R. V. Iozzo, D. K. Moscatello, D. J. McQuillan, and I. Eichstetter (1999)
J. Biol. Chem. 274, 4489-4492
   Abstract »    Full Text »    PDF »
Serum sErbB1 and Epidermal Growth Factor Levels As Tumor Biomarkers in Women with Stage III or IV Epithelial Ovarian Cancer.
A. T. Baron, J. M. Lafky, C. H. Boardman, S. Balasubramaniam, V. J. Suman, K. C. Podratz, and N. J. Maihle (1999)
Cancer Epidemiol. Biomarkers Prev. 8, 129-137
   Abstract »    Full Text »
An antiviral soluble form of the LDL receptor induced by interferon.
D. Fischer, N Tal, D Novick, S Barak, and M Rubinstein (1993)
Science 262, 250-253
   Abstract »    PDF »
Astrocyte mitogen inhibitor related to epidermal growth factor receptor.
M Nieto-Sampedro (1988)
Science 240, 1784-1786
   Abstract »    PDF »
Role of Intrinsic Protein Tyrosine Kinase in Function and Metabolism of the Epidermal Growth Factor Receptor.
G.N. Gill, W.S. Chen, C.S. Lazar, J.R. Glenney Jr., H.S. Wiley, H.A. Ingraham, and M.G. Rosenfeld (1988)
Cold Spring Harb Symp Quant Biol 53, 467-476
   Abstract »    PDF »
erbB-2 is a potent oncogene when overexpressed in NIH/3T3 cells.
P. Di Fiore, J. Pierce, M. Kraus, O Segatto, C. King, and S. Aaronson (1987)
Science 237, 178-182
   Abstract »    PDF »
Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene.
L Coussens, T. Yang-Feng, Y. Liao, E Chen, A Gray, J McGrath, P. Seeburg, T. Libermann, J Schlessinger, U Francke, et al. (1985)
Science 230, 1132-1139
   Abstract »    PDF »
Expression cloning of human EGF receptor complementary DNA: gene amplification and three related messenger RNA products in A431 cells.
C. Lin, W. Chen, W Kruiger, L. Stolarsky, W Weber, R. Evans, I. Verma, G. Gill, and M. Rosenfeld (1984)
Science 224, 843-848
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)